XB-CLONE-3545503
Clone Name: IMAGE:7717461
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Gene:
XB5769266
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC160520 [+]
ctaataataattaaggacaataaggaaaggcataggaagaggaacaactggaagaaatataaagtgcttattgtaaagaagcagaacagccttcatgagc
cttctatcccagctctgcccatttgccttggggtgtaatgtcttcacactgggcatcatcttcacccttctgcttctgctgttggatttcatgaagcgca
ggaaaccctgcacagacttccctcccagccccccctcctggccctttgtggggaacctgctgcagatggactcttcaagcctcagtaacagcttcagaca
gttaaagaaacaatatggagatgtattcagcctgcagttctattggcaaaatgtggtggtgctgaatgggtatgaggcgatcaaggaagctctgctccag
aagtctgaggattttgctgatcggcccccatttgaactgtatgaaggcatcgggttcactggaaataacaaaggggtagtgacagcaaaatacggccaat
cttggaaagacctaagaagatttactttgtctacactgagggatttcgggatgggaaaaaaatccctggaggagagagtgggagaagaagctgggtacct
gtgtgatgccttcctgtctgagcaagggcagttatttgacccacattataaactgaatactgcagttgccaacataatcagcttcattgtttttggtgat
cggtttgactacgatgattacaagttccaaaaattgttaaatctgaatcaagcaatgtttgaagttgaatccggaactatggcccagaaaattatctctg
accatcagaaaacatggaatcctgcatgcacaagggatctcatagatgcctttacgcttcaaatggaaaaggcaaaaggagacaaagagaatcattttaa
tgagaagaatctgttgtttactacatttgacttatttacagctggatcagaaacgtcaaccaccaccctgagatgggggctgctgtacatgttgcaatac
ccagatgtccagagtaagaatagcatggattgtcctggttaaataataaatggagcctcacaaagtttgttgtttcctgagtcctgcctctattggggct
atggatacagtgcggtgttctgggctagtgatgagagaaatttttcaccaggttttgccacaaaataacgcttatagactccaacgaccataaaacaaaa
gttgcatggcaaaaaaggcccattgactttaatgtgtttcttaatgtgtttttttgcagatttgtgaatttcctgtgaagtgaaaaggaacagattcact
tattactattctgggctaaatgtcactctttaatataagccttccatttgcagaacactgtctaaagcacaggctttggcaattgcagcatacattacaa
tagttttagaggtcaataagcatctactcccacgatcatcttgacagattagtacatggtgtagttttgctattgtgcaaaataggagatctcctaaatg
tcagtccttttccttcccatgtgtttttactaattcaggaaaggttcaagaggaaatagacaaggtgattggaaaaagcaggaagcctgtgatggcagat
gttttgcaaatgtcatatacaaatgcagtaatccatgaaatccaacgttgcgcagaccttgtccctctgtctttaattcatatgacatacagagacacgg
aagtccaaggcttttccataccaaagggtgttgctgtcatccccaacctgtcctcagtgcttaaagatgaaaaagtctgggagaaaccattccagttcta
tccagagcacttcctggatgcggatggcaaatttgtgaaacaagaagcatttttaccattttctacaggtcgccgagcttgcctaggggagaggttggcg
aggatggaactttttctcttcttcacgtctctcctgcagcgcttctcgtttcagatccctgacggagagccttgcccacgggatgatcctattgtttata
tagtgcagatcccacacccttataaactctgtgccaagattagataaattacattgcattatgtttctcatatacaatatgatgaatatgcattataatg
taaatatggcagtattaattatttacattttgggccggaggggggcagattaa
5' EST: CX978668 [+]ctaataataattaaggacaataaggaaaggcataggaagaggaacaactggaagaaatataaagtgcttattgtaaagaagcagaacagccttcatgagc
cttctatcccagctctgcccatttgccttggggtgtaatgtcttcacactgggcatcatcttcacccttctgcttctgctgttggatttcatgaagcgca
ggaaaccctgcacagacttccctcccagccccccctcctggccctttgtggggaacctgctgcagatggactcttcaagcctcagtaacagcttcagaca
gttaaagaaacaatatggagatgtattcagcctgcagttctattggcaaaatgtggtggtgctgaatgggtatgaggcgatcaaggaagctctgctccag
aagtctgaggattttgctgatcggcccccatttgaactgtatgaaggcatcgggttcactggaaataacaaaggggtagtgacagcaaaatacggccaat
cttggaaagacctaagaagatttactttgtctacactgagggatttcgggatgggaaaaaaatccctggaggagagagtgggagaagaagctgggtacct
gtgtgatgccttcctgtctgagcaagggcagttatttgacccacattataaactgaatactgcagttgccaacataatcagcttcattgtttttggtgat
cggtttgactacgatgattaacagttcccaaaaatgttaaatctgaatcaagcaatgtttgaagttgaatccggaactatggcccagaaattatctctga
ccattcaaaaacatgg
3' EST: CX978667 [+]ttaatctgcccccctccggcccaaaatgtaaataattaatactgccatatttacattataatgcatattcatcatattgtatatgagaaacataatgcaa
tgtaatttatctaatcttggcacagagtttataagggtgtgggatctgcactatataaacaataggatcatcccgtgggcaaggctctccgtcagggatc
tgaaacgagaagcgctgcaggagagacgtgaagaagagaaaaagttccatcctcgccaacctctcccctaggcaagctcggcgacctgtagaaaatggta
aaaatgcttcttgtttcacaaatttgccatccgcatccaggaagtgctctggatagaactggaatggtttctcccagactttttcatctttaagcactga
ggacaggttggggatgacagcaacaccctttggtatggaaaagccttggacttccgtgtctctgtatgtcatatgaattaaagacagagggacaaggtct
gcgcaacgttggatttcatggattactgcatttgtatatgacatttgcaaaacatctgccatcacaggcttcctgctttttccaatcaccttgtctattt
cctcttgaacctttcctgaattagtaaaaacacatgggaaggaaaaggactgacatttaggagatctcctattttgcacaatagcaaaactacaccatgt
actaatctgtcaagatgatcgtgggagtagatgcttattgacctctaaaactattgtaatgtatgctgcaattgccaaagcctgtgctttagacagtgtt
ctgcaaatggaaggcttatattanagagtgacatttagccca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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