XB-CLONE-3545489
Clone Name: IMAGE:7717319
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Gene:
nup205
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978677 [+]
ctgacctccagcacggcacagcacttacaggccgccggacaggtcttgcagttcctgatcgctcattctgataccatccaggcaattctccggagccagg
aggggagtctgggttccttgcaggagttggctttgcttacaggcatcatcagcaaggccgcactacccggtgttctcaacgagctcgatatcggtttaaa
cgaagggtccatgatggagttacaaggacatattggccgcttccagcgccagtgtcttgcgttattaaaccgctttggggggtccgaccgacttcggcag
ctcagtctgcaggatgattcctcccagccggacggtgtgagcaagagggacgacatggagttggccatgcaacagatctgctccaacgtgatggagtact
gccagacgcttatgatacagaactcgccaagcttccagcagactgtgtgtctcttcacccccaacctcacggagagctccagccgagatggaacacggca
ggattcccaggtgtccattctgccgtcatggcggttgccaggcttgggtatagtgatccaccttctgaagcagagcgccaataacttcttcacttactat
gatatccaccggcagagcgtcagcaagctgcagaacgtggagcaactcccgcccgatgagattaaagagctgtgccagtctgagatgccggttggcgtag
ataaaatctcgacgacgcagaagtacgcgctggcacggcggcgactcgtgaagcttatcaacagcagagccaaactgctttctctttgtagttatatcat
agaaacgtgtctgttcattctgtggcgccacttggaacattaccttctgcactgcacccacagcgactcccaggaccccgtc
3' EST: CX978676 [+]tggagatgataatttagaaacagttgtgtgtgtgctttgtatgtggcccccggatcttcatcaggcccttgatactcgcaggaggccccggatccgcctg
accagcgcctggatgaagctgtgacgggatcgcaccttgcagtacaatgattcgatgtctaacagtctcttctgcagggactccccgaaactattggcgc
cttctcttagcagcatatcaatttcctgttggctgactttattctgtctcaggttcctcaggtccatgttaggatcagtgttaattgtatcttgaaacct
tctgttgccgaaggtcgtattcgagaagacggggtcctgggagtcgctggtggtgcagtgcagaaggtaatgttccaagtggcgccacagaatgaacaga
cacgtttctatgatataactacaaagagaaagcagtttggctctgctgttgataagcttcacgagtcgccgccgtgccagcgcgtacttctgcgtcgtcg
agattttatctacgccaaccggcatctcagactggcacagctctttaatctcatcgggcgggagttgctccacgttctgcagcttgctgacgctctgccg
gtggatatcatagtaagtgaagaagttattggcgctctgcttcagaaggtggatcactatacccaagcctggcaaccgccatgacggcagaatggacacc
tgggaatcctgccgtgttccatctcggctggagctctccgtgaggttgggggtgaagagacacacagtctgctggaagcttggcgagttctgtatcataa
gcgtctggcagtactccatcac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: