XB-CLONE-3545477
Clone Name: IMAGE:7717511
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Gene:
mhc1-uaa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978685 [+]
ctcctgctcactttgggggtatctgcagtgtattctggcagtcattccctgcgctactattatacagcagtctcagatcgagcctttgggctcccagaat
tttccacagctgggtatgtggatgagacacagattgaaagatacagcagtgacaatggaaagtctgaacctgcagctcagtggatgaagcagaatgaggg
tcctgaatacagggacgaacaaacacagatcctcaaaggcaacgaggctgtgtttaagcacaatgtaaaggttgcaatggatcgcttcaaccagacctca
ggtacccacatcgttcagagcatgtacggctgtgagctgcgagaggacggcagtacccgggggtatgagcaggatggatacgatgggagagagttcctag
cgctggatacagagagatgggtgtatgtaccttccatgcgcgaggcgcagctcactacccagaagtggaacagcccggaggttaatgagcctgagagaga
gaggaattacctggagaatatctgtatcgagtggctgaagagatacctgggctacgggcaggcagagctggagcggagagttcgccctcatgtaaagatt
tcagatcgccaatcagatggcaatacagagcttcactgccaggcgtatggattctatcccagagaaatagatgtcaaatgggtgaggaatggtaaagatg
atgtacactcggaggaagccaaagagatccttcccacccctgatggcagctaccagctcagagtcactgctggaggtacacc
3' EST: CX978684 [+]ggcgagaggcttacagcaggcaaatttttaaattcaatgaaactgttgcttctactcctgttcacaagaaaggctcttggttaaggtttctattcaaaga
aaaatgtactctgatgttaattggaaattttctttgattcctaaaacataaattttctgcataagaggaactttgatattgctttctttatgaggatact
ttccttagacaacattagaccaatcactgttgtgttatgaccgctatatgtggttccactgaaagtgacatggtgtctcccatcatgatgggcatttcca
gtgccataactacatattactgggccccacagcaaattcagtgtagggccccagacattggtgagttaactattctactaaggtctattgaagtcactca
ttacttagagccctactaagccctctacactcttgcagggtctgcaagacagcccccaccctaggcacatgcttcttctgcctacccttagttctgccct
ggttcatatccagaacaacaccactgtatgacccacactgtgacctctgtaccacatgtgagcacccaggcttcaaccccccagcttttgaattgtaagt
tgaataagaggtggcatcagccccagctataaattttctcaagggttttcattctttcataatgtcagccatagtagggaacatttacttttatcctgga
ccagttactgaacaacagttccctccaatccactgacaactgtatagagacgcagagaaccaaagtttccagttctacaatcctggaacttgtcatgcct
ggaaacagaagcagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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