XB-CLONE-3545443
Clone Name: IMAGE:7717324
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Gene:
slc51al
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978709 [+]
ttatttaggcatgacaggagcactcaaacagtaataacaccacaataaaaagtcagcgagctgcagatattgccattaatgtgctcggcatacataacaa
ctatgattattttgacagctcctctaactgaaccatcattcttaatccagttggtagtatggcttaattattctggaatctttggctctgtaagtaacag
tagggtttcccataatacatagttatttcactgtggcatcatagcaagcacagcgagtctaaaaacataatttacactcgtataatctgaagctgtttga
gcaactaaaaacaaggtgctttctaagcaactatacaattggttgctgtgaatgtttaattatttgccattctgttctgcatctgtctgtgacaatgatg
tttaactgcccctttgctgaggtggaagacaaagggagtccaaagcagacaggaggcctccgctcagaagcacgtgtgtttggaagtaaatagatggatt
gaaacccaggcgaacagtattatggcataggcatactcactcccatacaataacatttattagcaatataaatacattatcatttattaagaactcacaa
ggtttctccaaatacttaacaagtatgctcaaattgtatacatacaagatatatatgataactctatagcccccacccaaaaaaagtgaaatgtccaagt
tcaatgcagtgtttgttgttgttacccagctacatatgtacaaagtagagcggcagcccacccccttagtgcgctagtatggttgccactgctcctggag
gtcactgtgcttgaaagtatcacttcactactgtgactactcggca
3' EST: CX978708 [+]aggtgtgttccttgcagtgttaatacacaagtttcagctcatgctggtaaatgaatgtgggggaagaagagaatttctctcaacatttggtgatacgaag
ctgaagattagcactggccccttctgctgctgttgcctgtgcctgccacacaaagacatcaacagaaagactttattcatcctgaagctgggtacatttc
agtttgccttcctacggccggtgcttatgtttctggctgtggttctttggacaaatggaacttatatgattggtaattcatctgcagaaaaagctactat
ctggattaatattggagttggtattaccacaataacagccctttgggcagtgggaattatgtttaatctagtaaaggacaacttaaaggagaaaaatatc
attggcaaatttgcagtctatcagtttacagtcattttgagccaactgcaaacctcaatcattaatattcttggtacaaccggtgtcatctcttgtgttc
ctcctctcccggggccatcacgggcttcctacatgaaccagcagcttctcattatggaaatgttcctggtcacagtcatctgccgagtgctgtaccggag
gagatatgatgataaaaatcttttggaaaaccaagagaccaatgataatttacgaaacagcatgatgcatctgaatggcaaagctttggaagatggaccc
caaagtgtttaaagggcaccaagacacattataatctcagtgtaccaactgcaaggtgtagtggcatctccagaaatgctacccctggtctgacaaaatg
tgcagtgaaattgtgaccttgtgcctctgccaatgcccaaccagaatgtacagtgcagctgctggacattctaatgctaaactg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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