XB-CLONE-3545416
Clone Name: IMAGE:7717374
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Gene:
apoe
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978727 [+]
gatcctcttgctcacgcttgggctcctggcaggggcccaggccagatctctcttccgagatgcaccaaagacaaaatgggagactgccctggatagtttc
tggctaacagtcaacaaaatggaggaggcagctgatgaggcgagagccaaaatgaaggattcaccaatcagtaaagagctggatggccttatcaaagaca
caatggaagaactgagcatttatgcagaagacatgaaaagtaaggtgagccccatggcccaggatgcacagcagcgctttactgaggaggtatctgccct
ggctgataagctcaagtctgacatggaggatacaaagaacaaagccattcagtactcaggagacctgcgcatgatgtttgatcagaacattgaggatgtc
caaggcaaggtgaacatgtacatgaagaaactgaagaaacggctgagtaaagacagtgaggatttaaaaaagaagctcagccaatatggagaagatttcc
gtaagagtactgaagacaaagtggaaaatctccgtaaagccgtggagccttatttgtccaacgtgaaagataaagggcagcagaggctgatgaacctgaa
ggatgccatagatgagcaagggaaacaactcagagaccggtttgtctctgttgccaaagacgcccaagctaaaatgaagaagacagcctctgatgtgaaa
agcagtgtggataaactaggggaacagttccgtaaatggttcaaccccaatgtagagagcctgcgcaatcagcttcaagccttggctaagagcctgcaga
ccaaggccaacaaataaatgaa
3' EST: CX978726 [+]cccagtaataagatgtattttatttttttttatttggataaaaattgcattgaaatagacatatattttacagcagagaggcactttataaagtaggtaa
ggcagcacatacagcagaaatctagtgaaggcacaatgttattttaggtttaaaaaaggggagtgtattaagaaacataaagacaccagcaatgcccata
atggtgccctacattttcaagagctggtgatacaatatggatatttagtaggttgagatacagacatccatgaaccctcttgtttaagaccccagctgtt
tggtacaagtgattccttgcatggcctagtttactgcttgcccttggggcctgtgttccaaatggaaaatataacatgtagaggctcatatacttttctg
tcttagcttttctgtgaatcttaacttgcctaaggtatgtaggttcttgttcagacagggcagtcagctggagatctgtaggacacaaggcatgtttagt
gcaggagaatatgctggggatagtttagtgctataatttaaggcttgcccttaggttaaatgtgcctttaatactgaagttccctttggctttacatcgt
tcacctctaatttgtgcttgtcattaatgtcagagatctgattaatgagcttgcctaggtgagcagtataatcactgggtgatgtacctcagatacaaac
agggaaaggcaagatgtttaggcatgttggatccttaatgtcctgctccacctcagggcgcaatcttatggtcaaaagtagcagctgcaagtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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