XB-CLONE-3545363
Clone Name: IMAGE:7717140
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Gene:
znf782
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978763 [+]
ctactgttccctctcatgttagtattacataacggagagttagcgctaagattactattagcctgttctacttgtaacagagggatctccttagctggta
acggtatccccctccccctcactccttcccatgagcccttactaatcccactgccaggtctgcactgggatccccataaataacagtctctatccttccc
tttagcagcctgtgagtataaagatgggagcgatgttacagcacatacggaagcaactttatgttgtaataatgatgggaatctcacaaaccctgatgtt
tctccagcggaacagcccccaccggccaatgggattaaagaggaggtggcttcatgggaagggggaaaccaatcagattgcagcattaatccccttacag
aacagatacagggaacagacacgcctactcctatcatggggtgcagcctgaataacagctcggcagaggattatatatcagttgttattaaagaggaaga
atcttcaggggaaggggaaacccaatcagattgcagcattaatccccttacagaacagatacaggtaacagacacgcctactcctatcatggggaatagt
ttaaaaatggagcgtaataaaaatgatgaaaattcccaccaatctccacataaatctcacataacagaaaatacattaagtgggaaatacagctgcaaag
tgtgtcacaaacactttagtcataagagagacttaaatanacatcaaagaacccacaataaaccctattcttgttctaattgtggaagatgttttataaa
tcgatccgaccttaaacgccct
3' EST: CX978762 [+]ctgacacgtctcatgaaaaagggcttttcacaaaactgatttaatgtaaagagaccctttataggataatggtgtaaccccctttcctttcatctcaaca
aatgccccattggaaatgacattgggttaataatgacaagatgatgttataggctttatatttacccagtaagagcctatgtacccggcaagtcattccc
agctgggtatatccccattatgttacctactgtgtgagcattagggaacagcatttgcccaagtgataccctggggggctcgtaccccttggaatatgtc
ccttttttgttcaaagaaagccagaaaagtatatgttggtgggaggcggagatatattccagtgtctgtgaactaagaatgaaattaggactcctcagtt
cccaatgctgagccagtggttatggtgcattctgctttggtgcaccacctgtcttagcattacccagtgtccatcaggagtctttaccaggagtctttta
gcctcattcggaaaacaagtttacagtttttctcatgtgtgaaagagtatttttagactactgttcaatacaaatcatttccctcattcagaaaatggtt
tctcccctgtgtgagtctttcgatgcttataaaggtgagagttctttgaaaaacatttcccacaatcagaacaagaaaatggtttctccccagtgtgggt
tgtataatgatttttaaggtgtgcatgggttgaaaaacatttctcacaatcaggacaagaaaatggtttctcccctgtgtgagttctctgatgacgatca
aggttagatcgacttgtaaacatttcccacat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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