XB-CLONE-3545348
Clone Name: IMAGE:7717380
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Gene:
tagln
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978773 [+]
actgtatgcaaaaactgtctacaaaatgtttagtacattagtttttaaacctctttggggacttaccaatagtggatatgcagaattcacaccatccaac
ttcctttgtggatacattgaaaccatgcaactgttgctgggtccctgagggtttgaagacacataattcagtaggtagtattgcaagcttacaatagaag
ctaccaagctggctacttcagaccccgaggaatcaacagctggctggctgtggaactgtttttatgagttatacagccataggcatggccctgagaatag
agcacatcaagacaaagtcacatacacagacagccagctcactaacaaatctattgcaggtagggttggcacctggctggtattttacagacctggccag
taaaaataggccaatgttattaatagggaaaaataataaaaatattggaattggtatttttttccagaaaaggtgacaaccctaattgcaagtgaagttc
tgttgactacacatattcatttacttaagccttgcagtgctgtctacttttctactgactggctttgatttcatcttctctgcataatttaataaatgaa
tttgttttgtaattgcaaaaattgtctccccaagtgtagcctccttgtcttcactatacaatgtagtcacctctctatagcatattagaaatcacatctc
atcactgcaccaccccctgtcaaacgcctaaaactgaagagttcaatagttgatttaggaatacagcaagctggcactgtgactgcatcaatgaaaacta
caacaatctgacataccttaaccaca
3' EST: CX978772 [+]gcaacatcaaatatttaaacttattattgcatatttggtgtaaaatacacctttaagtgccagtagatgtacagaatggtttcttttttatttagaatgt
ctaaaaaaaaaaacacatgaaccccattagcagacctagcggggatgtattgtgtaattatctgaaaaccaagtttcacattaatactgccctctgattg
gacatgcaactgtatcacttaacaagcatcaacatcatgtttctggttggcttcacagcttgctccaatcacattgccatgaccaggaagcttgatgtgt
gctaggaagcctgggtgggaaggagctaacctagcactactcactactaccaagacaatgttaattgtaaaggtattttacaacaaatcacaactaataa
agtttatttttgtaattctgttggtttccaattgactaatttcagatttaggacacactagacattttattgcagtgatcaaacgtcccttaatctctgc
caactaattcccataaaagtgtatggggaaaaaactgttggatcaagagtattatctgaaatcctagtgattataatctgatgtctgggttctttggtga
tgtgcactttggagtggcattagcagtgggattcaatacttgtacaatctgttacctgtgggttaaggtatgtcagattgttgtagttttcattgatgca
gtcacagtgccagcttgctgtattcctaaatcaactattgaactcttcagttttaggcgtttgacagggggtggtgcagtgatgagatgtgatttctaat
atgctatagagaggtgactacattgtatagtga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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