XB-CLONE-3545264
Clone Name: IMAGE:7717340
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Gene:
apool
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978831 [+]
ggtttcacctcttatggcctttatgcagtttcagaccgggagcctaagggaaatctcctgaaacctattcagctatctgtttattccgttcctgcacaaa
aatctaaatatatcgaagaacaccctggccgtctccagtctggattgtcttcagtgagggaaactatttggccttatgtggtatggggcaagcatttttg
ttcatctgtcagaaatggagtagaagatacagtacagtttgggaaagatagttatgtttacctgaaaaaccctcctccagaatttcttgcaagattgggt
ataatcacagttagcggtttagctggtttggtcttggcaagaaaaggatccagactaaagaagattgcttaccccctggggcttacaacattaggtattt
cagtttgttatccaacacaagcagtcatttttgcaaagttaacagggcgaaagatttatacagtaagtcatcaaacatatgatacattgagttccttgtg
gaaaacaagcatccacaaagaagataaacctcaaacacaaacagaagatcttaagataccagtttcagtgacagaaggggaccattatcaagggaatcaa
gaggaagttttggaaacactgataccggacctgactaacagtactaagtatgaggacccagaacccttaattgctgtggagactgaacctcctgctctta
taaatcaagaaatacttgatgtaaatgaagaagttaatttaaataaagagggactgaataatcaggagaaacagacaactggctcttcagatttaaaaaa
tcagcggaag
3' EST: CX978830 [+]gagaggcaaaaacatgggggtagaaattgaagtgatgacaaacacaaagctttgaagtgacatgtacagagcacataacatcagtgttctgtaaagagtc
ccaaataaagcacttaagggttaaattagattaatttaatttagtttctggtgctatacatatctacatcttcagggtgtgcctgtccatggtccagaag
gcttgggtcaggtttaaacttccgctgattttttaaatctgaagagccagttgtctgtttctcctgattattcagtccctctttatttaaattaacttct
tcatttacatcaagtatttcttgatttataagagcaggaggttcagtctccacagcaattaagggttctgggtcctcatacttagtactgttagtcaggt
ccggtatcagtgtttccaaaacttcctcttgattcccttgataatggtccccttctgtcactgaaactggtatcttaagatcttctgtttgtgtttgagg
tttatcttctttgtggatgcttgttttccacaaggaactcaatgtatcatatgtttgatgacttactgtataaatctttcgccctgttaactttgcaaaa
atgactgcttgtgttggataacaaactgaaatacctaatgttgtaagccccagggggtaagcaatcttctttagtctggatccttttcttgccaagacca
aaccagctaaaccgctaactgtgattatacccaatcttgcaagaaattctggaggagggtttttcaggtaaacataactatctttcccaaactgtactgt
atcttctactccatttctgacagatgaacaaaaatgcttggcccataccacataaggccaaatag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: