XB-CLONE-3545247
Clone Name: IMAGE:7717246
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Gene:
slc25a3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978842 [+]
aaatctttaaccatcagtttgttaagtttaaattacataaatgcaataactctcatctgtttatttcaacagataaatacagcggtaaaaccaagttaca
atgtgtaggctatgcggcaaggtgagagcacaaccaataattacatctgaaacaaccataggtcctattttttagttgaatttataaaaaacgccttttg
aaaatgttggagtttgcatgatttgagtccatgtttactgagtgagaccaagcttcttcttcaaggattctggcatctcagggggaggaggacgaggaag
tctgaagtaaaccttcacagagtcatagataaaccactgcaaagcagtcaaggtaccaatcatgatgatacgggcagtcaaacccttccagacaccttta
ggcccaagtctcttcagaacttccaaagcagtgcttcccttttccttgttcagtacagagaccacagaatccgcaggatgtgagacaatggcacagaaga
caccagcaatatatccagccacgaaagtgacaaccaactgctcagattttgaacattcgcttctgggtttgggcacaacatacttatagagagcttcaac
agttcgttcaaagcaagcaaatttcatcattgtgtatggaatctgcctcatccaaagaggagcaacacctttgtaaaacgcccagatgccttcctcagca
tacatcttgggagcagcttgtcttanagtattagcatagcctggttgtgtctgaatgcgtacttttgcagcttccattggagccagagcaatatcagcaa
agaactcagcactggcagacgcagccaggtaaaagagaagtacgccccatgntagga
3' EST: CX978841 [+]tagcgcggaccaaccctttcaatgctccccactttcagttggggcaagattctgtaatatcaaggaaaggcaatatcccccaaacaaagccgacccgtca
gcttgctgctgctgctgttcaagaagcaggtgaattcagttgtgaatatggctcaggaacgttttatgcatattgtggctttggtggcatcctgagttgt
ggtctaacccacactgctgtcgtaccactggatttggtcaaatgtcgtcttcaggttgaccctcaaaaatacaagagcatcttcaatggcttttccatta
cccttaaagaagatggtgttcgtggccttgcaaagggatgggctccaacgttcattggatattcaatgcagggcctgtgcaagtttggtttctatgaagt
gttcaagatactgtatgctaatatgctgggcgaggaaaattcctacatgtggcgtacttctctttacctggctgcgtctgccagtgctgagttctttgct
gatattgctctggctccaatggaagctgcaaaagtacgcattcagacacaaccaggctatgctaatactttaagacaagctgctcccaagatgtatgctg
aggaaggcatctgggcgttttacaaaggtgttgctcctctttggatgaggcagattccatacacaatgatgaaatttgcttgctttgaacgaactgttga
agctctctataagtatgttgtgcccaaacccagaagcgaatgttcaaaatctgagcagttggttgtcactttcgtggctggatatattgctggtgtcttc
tgtgccattgtctcacatcctgcggattctgtggtctctgtactgaaacaagaaaagggaagcactgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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