XB-CLONE-3545191
Clone Name: IMAGE:7717490
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Gene:
dda1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978882 [+]
ctcaatgagagtaatttcagtagattccatgcagactctgtgtgtaaagcctcaaacagacggccatcagtatacctccccacaagagaatacccctctg
accaaatcatagtaacagagaaaacaaatatacttctgcgttatctacaccagcaatgggacaaaaagaatgctgcaaagaaaagagatcaagaccaatt
agaaattggagagacgtcggctcccccacgaaagattgccaggacagacagccaagaaatgagtgaggacacataggcgccttttgcaaaccttccttta
tcacctcttgtcccactccaccgtgtccctccctttgtacacaggatggttctccgatgtgtcgggagcaacacagcctttcctcgccctccaaccttgc
cttccctttctcttatggtccataggagccaaaattgtatttatttcttccagattttatttatgctgcgctgcaaaccgttcaaagagaggatagtgtt
aaaacgtgaataaacttttttttttgggttaactttttttttggtctcccccaagttgctgttgagtttggcgtgtacaggtttatttgccccactggtt
agaggaggaaactctaggttagatcagacaaacctgctttttgcatctcttgtagtcaatgagaatatgtgtatatactttttttttttttattttttgc
ataaaacacctgtgcacagctattttattttcccttgtcacccgttggtactggctttccagtcagtgggtgtttctttgcagaaaaggcatattcactg
tgttccactgttactagagctgatggccatagcattggg
3' EST: CX978881 [+]tccgacctcacattctggtgttggaaaccatcagcgcaacaaacgccggcattgtttaaacatcacgtataacagtcatttgttgcttgcacacttggat
acaggtgcttttttgttttttgttagctgacaagacaggttccctaaatatagaattgtgacgttgactacacttaataccagcctaacatattgattat
agtatgtagtgatggagtcacctctgggatacgaggcagcctatttatggcacagcagtaaatcttcatatgctgaatgcatggactcctacaagccact
ctattcccaatgctatggccatcagctctagtaacagtggaacacagtgaatatgccttttctgcaaagaaacacccactgactggaaagccagtaccaa
cgggtgacaagggaaaataaaatagctgtgcacaggtgttttatgcaaaaaataaaaaaaaaaaaagtatatacacatattctcattgactacaagagat
gcaaaaagcaggtttgtctgatctaacctagagtttcctcctctaaccagtggggcaaataaacctgtacacgccaaactcaacagcaacttgggggaga
ccaaaaaaaaagttaacccaaaaaaaaaagtttattcacgttttaacactatcctctctttgaacggtttgcagcgcagcataaataaaatctggaagaa
ataaatacaattttggctcctatggaccataagagaaagggaaggcaaggttggagggcgaggaaaggctgtgttgctcccgacacatcggagaaccatc
ctgtgtacaaagggagggacacggtgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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