XB-CLONE-3545086
Clone Name: IMAGE:7717308
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Gene:
ehhadh
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978953 [+]
agaaaatcggaataggaacacctacaaattattgttatcaacaattcaaaggtggtatttatttgtcccagagagagtgatttagtgtctacaaatgtcc
atatcagtgggacagcttaatcacctttagggacagagtattgatggataaaactgctgctgccaaatgggcatcaaataaacaacagaagcagttatga
aagccaactgccttgcatgcagcttctttgtgacaggtaaaccaaataggctggatgtcactgataatgtacttgcaatcccaccaagccactgctttct
gcatttaattttgactttaatagtacagaatacagctaaagcaagatgccttctgactgcagctgtgggttgcatccagcctaccttgcactgaagctgc
atcccataacaaggagcttttcatcctgtaatatacatctctattggggttatgtaataaaaggcactagttttgcccaggagcagtaatccatagcaac
taatcagcaggtagaatttactggtcacctgcttaaaagcaaacatcttattggttggtatgggttactgctcccaggcacatttaggattatgctggat
acgtatagaatgatacagtcattggaagcaattttgactcgtagctgaaatctaccatttccttttaccttcctttcacacttttcagaattttctttcc
agaaaaaggttatggctaatgccatagggttcattctcgacctgtgaataaatgcaggcagagaatcagtctctatggtggctttagcctttccttgttc
ctgtacccagagccatggtgtcgacccagatgcaagcaggtggagtggattttggtgccaaaatgcatagttgcaagtttca
3' EST: CX978952 [+]attcacatagaatcaggtcagcaatttgagaacgatcattttcctttctggctaaaatgcagtttatgtaagggatgtgaaatcattttaacagcaagat
gtacagaacaaaaagtaaaggttttttgcttttttttttttttcttctctctattgcattgaacattttgagaagtgttgacgctggcaagatcacaggc
attgagagcaagcagaggaagacggtacattcagtgcactcatttaattatgtccctgtgctacggagggttcactgctaaagcactcagcttaccgggg
aagtcagagaatattttcttttgtagtggaaaatacatggaatttttcccttagtaatgtaagtgactgacattacggagccttaatgatatcaacagga
aaaaagatagtgactaagttgcaacctagcggtttatgaaaatgtttaacacaatatactttagagccacacccggagggctacacaattaaatagctct
cattcagttaaatctctatggtttttatgtgctcctgcaatgtgtgcaccattatcattactatttatgcctttgtgtattaaagatgtatttatgccct
ttgtcagttactccatatctaaagctggacacacatggagtgagatggctgaccaacttggtagggttttaaatttgtttttttttggttaatgccgcat
tgggctgatccttggtctgtggactaacactggctgacattcagcccctgcactagcatcatcctttccctgtgccctcacccagaatcagtgtcagccc
tgttac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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