XB-CLONE-3545083
Sequence:
5' EST: CX978955 [+]
atttacacagatttacactcgcgtacgataaacactttttaaaaatacttttttctcaaatgcatttcgcagtatttacaccagtgattcataaatgttt
ggtgttgggtccccatactgttcccagtgagtctctaaaatatcctccccaaataggcgaatgggaaattaacagaaggcgcatcattctaaagcaattc
ctgtctccgacattttatttgggtcccctaataaaaggaaatatttttataagtacaggatggaaataactggagggtcaatacaaatgaataatgttga
tgttttggtgttactgggcctttaaccaaggagagtttgtctcagagggaggccccaggcccaggatagatggtgggatcaataggaatttccagcccca
ggggatgcgctacggcggctgcaggtggggctcagaaggaagaattgctttgtgttagtgatcgcatcttgcgcatcttctttttatacgtcatggacat
tgcgatgccgacaatcgcacaccctacagcacatccgccaatggtgcccactatcactgaccagtagggcacctggccttcttcttcctctggcacggga
acaacagtttcagggggttcagggagcccactgtttttcagggtgaagagataaggcttgtcctgcgtagggcatttcagatttgatcgatcgttggtga
aattattgtattcctgtttttgtccaacgggttccagcatgctgttccacagggccttggccatctctgactggcctctctcggtgaaatggaagcaatc
cacggaaa
3' EST: CX978954 [+]ggttcagactgagtttattggccacatgtaaatggccaaaccaacagcctggccaatagattcagctgaagaacaaggaaatgaattgaagtggatctta
ttgtcacagagcagagcaaatactgacctcattatgttccctctttccctcaggatcagccgttcctaaggacatacaaaaacagcaattacaattatac
ccctgcacagcccacccagccccctataaagaactggggcagtgacctgacatgtcaggcaaccggaccttcctcccaagtcccaacatcagtgcacaag
ttacgcccagctgacatcaaggtggtagcggcccttggggactccctgaccgcagcgtttggcgctaagtcatcttcaatcgctgaattggggactgagt
atcggggggtttcttggagcattggcggcgatgggaccctggaggaatgcaccactctgcccaatattttgaagaaatttaatccaaacataaagggatt
ttccactggagtcggcagagagaacgaaatgtttaatgtagccattagtggggccaaagcagaaaatatggcttctcaagcaaccaacctagtgaataag
atgaaagaaagcaccgtgatcaattttaatgaagactggaaggttatcaccatttttattggcggcaacgacctctgccagttttgccacaacagggaca
gatattccgtgcacaaacacgtcagccatattaaaaacactttggacatcttatacaaagaggtactgtacgttatccgtgcaaaacactgatactcact
agtgagggaagggggcaagcttatgctgctggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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