XB-CLONE-3545077
Clone Name: IMAGE:7717452
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978959 [+]
tccatcgattcaattcggccgagggaaaaatgttgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatttattcccatcatcctca
agataaaaaggtgtcaacaggggcatgggagctatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctgactggttgtttcca
ccctgagagtgcgcttagctactctcccccagggtgtgcttgtcgaacaggtactcgcccatgccgttctgcggcaccccaaggcgcttcaggtttgtga
tgtagtctccaagctccttcatggccttcacctgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttgtcagatgccagctt
gtgcagatccagtaaagcctggttcacagtcttctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcacgctctggtttcttg
atatcctgaaggacggcgcggcccccacgtttgttttggtatttgaggaacttttcggcatgctccctttcctcatgactctgctccttgaagaacttgg
ccacatggtggagtgccacgtcatcacgatcgaaatagtaagacatggacagatagacataggaggcatacaactccatgttcaccatacggttgatggc
ggcttcgcagtcgctgttgaagttctgacgcacctgggattgcatggcggcgtttcggtgtttgctggtggcgcaaaaaagtcacaaaaaaaagagtaat
gcgagagagagagagattttggtcta
3' EST: CX978958 [+]ggcgagaggcttagaccaaaatctctctctctctcgcattactcttttttttgtgacttttttgcgccaccagcaaacaccgaaacgccgccatgcaatc
ccaggtgcgtcagaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatgtctatctgtccatgtcttac
tatttcgatcgtgatgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgccgaaaagttcctcaaatacc
aaaacaaacgtgggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagccatgcaggccgctctgcagct
ggagaagactgtgaaccaggctttactggatctgcacaagctggcatctgacaagactgatcctcatctctgcgacttcctggaatctgagtacttggag
gaacaggtgaaggccatgaaggagcttggagactacatcacaaacctgaagcgccttggggtgccgcagaacggcatgggcgagtacctgttcgacaagc
acaccctgggggagagtagctaagcgcactctcagggtggaaacaaccagtcagctctctgtttcaaaatactgtctctttactatctgtgtggattaca
ccatagctcccatgcccctgttgacacctttttatcttgaggatgatggggataaatctgtgctattactgttctgaatcatgcagaagctgctgtgctt
gtcaacatgtttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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