XB-CLONE-3544864
Clone Name: IMAGE:7717711
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Gene:
eaf1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979102 [+]
gcacacagtaatcactgactggcattagtacagtcaactatcaccttcagctacagaacgataacaaggcagacctacaaatgtggaactacagctccca
ggacttctaaatggagtttagctgtgaagtatagaactgtagttcatttgaaggatcactggttacctagcagataaattgcatagatttagattacagc
aattattgtgcttctcttatgtatgcacacatatttacatccaaaaagatacagggatgtaaattagctctcgataatgtacctagtatgtatgaatgta
ataaggacttagattgtaagctccactggtgcaggaactgttgtgaattatacatgttcgcaatgtaaagtgctctttatggtgtagttcaccatgtttt
tcccaatgcagtttctcagcataattgtgcaaaattgtatgcgtgtccctaggtatacgcttagtggcagaggtgtaaaacgtgctgatgatgcagaagt
tgtgctccgtatatttggggcaacatgcaggcaggcaccccttaattgcatgctatttagggtgtgcaatgcacaacagtaaccctgtacttaaagcctg
ggcaatgagaagtgtaaagtacagggctcccatgccaatgtgccttgttcctggttcacactgctgggttaaaggtgagcatttgcgctaagccaattgt
tttccatacaacattttaatttgtgcataattgactctgatattagggtcttgcatttccttctttttgtttcactcatttcacacataccatcattttt
atgagttaatttcaaacaaaggtgcatctgccagtaagtctgtatgtccttccctgtctgtatgc
3' EST: CX979101 [+]caaatgcactattactttattttgaagataaaatatatatacaaaagccttacataaaataaaatataaatgttcatgtaagaatgtaaaattttagcca
ataattaaatgtattattattattaccaaaatacatgaaggcatataatcttatgccaaacttacaaataaaatgtaagcttcttaaaaccagcataaat
aatgaaattagtataacttcagtacagagatacacacacacatgttgggacacgagttaagctacaagttggccaagacatcagtgggaaattgccttca
gtttgcaacattttctaatcatacttctttgccaaaagggaatgcagttccaattatataaggcaaagtgttgcaataaaaaaaaaggttctagccacaa
actcatgtgggtactctggtagacagatttgagactggatcacagaaagtctaccaaaacagaaaatgaaaaaaaggactgtccttgttatagctcatca
gctgatttatttcatatgagatcccataatttaacatgtgggcctacagactagatcatcccaaaagacccttatcactagcatgggataaaacatagct
taaaaataatatatcatggtgggggaaaaaaaaaaagaggaacaaggaaataaaacagaggaaaggataaaaggaatgggtaacgttattgcatcatatt
ttacactgcagagattgttaatgattcacatcactccctgcccaagcagaagcttacactctgatttaactatttacattcnctcaataaacacagacaa
actaacctgcctgtgtcagtgatgtaattactggaatgcggggaaaaaaacagaggtaaagcata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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