XB-CLONE-3544855
Clone Name: IMAGE:7717855
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Gene:
XB5960519
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979108 [+]
tttcaggttttatttaagtacctcaaaaaagtctgttagcatggccacaggtattaaatccccaatctagaaacccattaccaaaaatgctacaaattac
aggaacgttcaataacacacactgtgcaccctgaaactatagtgcagggcctttctcttctttcagaacaaattacaggaaggctacctcccatagagtc
caattttagcaaaaataacatatttatctttaaaggagaagttaagtcattttggcagtttactgccaatagatttgccactttagttccacctagaaca
ctacatttattctgcagaaactattaccatacctgattagacagccctagaagctttctctgtttgtgttagacagaagctgccattttagcttggtctt
tatagcttcctgctgtatagctctagccattatagctcagattacacattcctatgggagggggagggagttcttagcattcttatgggaggggggagca
ggagagaggaaaaaactgtgcagaatctggccccaggaataaaggatttttgtaagagaggaggtcagataccctaagaatatatttacaaaaaaaggag
acaagaaattctgtgtttcttttgatagaggaagcatttctgtaagtgcttatggctctatttacatagacctttctgataaagcttacttagtttttcc
tactcttttaaaaattaattccttttaatccttaacagaccagtagcttgtacttgatggtaactaagctgcattaatcaatattggtgacaaaacaatc
ctattgtgtttttttttagcagacataagatatggttatccaaattatgggatgatctt
3' EST: CX979107 [+]ttttatgctcctgtgagctcaaccatcttcatccatgccatttctgtgtgttctaagggtagcaggtatcgaagaaagaaacttgatgagataaaaaaat
attcgtttttagaacccacattactaccttgtattatatactacttattgataatgtatctaaagcgccaaaatattctgcagctgtatacattgaatat
acaacttgcatacaaaaacaagaactatacaagaggtaccttgtaatgattttgcagcttgcctcacctgcccataactgtaagctttgtggtgcaacag
aaggccagataactaaaggattttcttgatatattggattcagaagctgctgtcgctcttttggctggttaaggtgaccccacagcgagtgtgtccgatc
ttgcacctcatactgacatctggtgggaaaataattgtgactttttgaagttaatgcaataaagagggcaggtataggatctattgtccaggatgctcag
gacctgggttcctaatctaagatcattccataatttggataaccatatcttatgtctgctaaaaaaaaaacacaataggattgttttgtcaccaatattg
attaatgcagcttagttaccatcaagtacaagctactggtctgttaaggattaaaaggaattaatttttaaaagagtaggaaaaactaagtaagctttat
cagaaaggtctatgtaaatagagccataagcacttacagaaatgcttcctctat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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