XB-CLONE-3544816
Clone Name: IMAGE:7717763
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Gene:
fnbp4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979135 [+]
ctccatctcagacggcccagagcaagtctcaaccttcagaaaaaacaaagaagatcaaaaaagaaaaggttaaaaagagcaaggtgaagatgccgtccct
ggtccagaaatggcagagcatacagagggaactggatgaggaggaaaactccagctccagtgatgacgaccgagatgttgtcaaccagaaacgcattgaa
gaatggaaaatccaccagttaacaagtggcatggcagagaaaaacgcaaattttgaggcgctaccggaagactggagggacagactgaaaaggcgaaaaa
tggctccaagcacgtgaccctcctgttttgtacaactgtatattcccacaactgatactgtaatttctgccccttttataccagtgttaatgtgttccag
gtggagtagcacttacatttgttgaacgcaatggttcagctttgtccgttcagtcccaggattttatgtgtgcgtttctctgcagctgggaagccactgg
ggaaatcccactacaaacagctgcttttttgtagttgttgtgataccttctgagcaaagggttaagttaatgcaacacaaacgcactcactgttaatacc
gcctgtagcagattatccattggcagtttttccaccagctgagaagtgccggcttcctacggagtgtgacttcagcaggaaaagcaggcgcgatgcttag
tgctccagcctacgcttccatgtttttcctactaaaatgaatgtgagccgattgcaggcgtttctttacgggtggaaaatcccctgtgcgtgacatttat
acc
3' EST: CX979134 [+]caggtttttaagttgtactcgttacaaagagaatagactgtacttgaggtagtttccctttatctttgcttattgaataatggaagccaacaccctcagt
tataggtccctttactcgactgggggcagaatggagctcgggcaagcaaagaaagttagaaataatgctctactgtcccatcaccggggatttatgaata
aaaatatgtttaggaataaactattctcccaggtctttggggggtttgtagaatatataaaaaaaaccttctaaaggggaaatatgattttttaaaaaaa
aaaaaaaacaccccaaggttataatgtcacgcacaggggattttccacccgtaaagaaacgcctgcaatcggctcacattcattttagtaggaaaaacat
ggaagcgtaggctggagcactaagcatcgcgcctgcttttcctgctgaagtcacactccgtaggaagccggcacttctcagctggtggaaaaactgccaa
tggataatctgctacaggcggtattaacagtgagtgcgtttgtgttgcattaacttaaccctttgctcagaaggtatcacaacaactacaaaaaagcagc
tgtttgtagtgggatttccccagtggcttcccagctgcagagaaacgcacacataaaatcctgggactgaacggacaaagctgaaccattgcgttcaaca
aatgtaagtgctactc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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