XB-CLONE-3544783
Clone Name: IMAGE:7717575
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Gene:
cbr1.3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979157 [+]
acctgacagccagggaccccaagttgggagaagaagcagtccgggccctgaaggagcaggaagggctgtccccccacttccaccagctggatatcaatga
cctgcagagcattagggccctggggggtttcttaaaggagaagtacggggggatcgatgtgctcattaacaacgctggcattgcatttaaagttgccgac
accaccccatttggcacccaggccgaagtgactctgaaaaccaatttcttcgccacgagagatgcctgccatgagctcctcccactgattaagccccgtg
gtcgagtggttaatgtgtccagcatggcgagttacatggccctcgggcgctgtagccaagaactgcagaaggtgtttcgcagcgacaccatcacagagga
ggagttggtgaccctcatggagaagtttgtagaagatgccaagaagggagcccatcagaaggaagggtggccaaacacagcttatggggtgtccaaaatc
ggggtgactgtactgtcccgaatccaagccagggagctgaatgagaagaggaaagatgatgggattctgctcaacgcctgctgcccggggtgggtgagga
ctgacatggctgggcccaatgccaccaagtccccagatgaaggtgcagagacccctgtgtatttagctctcctgcccaacaatgcacattctccccacgg
ggagttggtgagtgagaagaaagttgtgccgtggtaaagcctttc
3' EST: CX979156 [+]tggcagatggagtgatataaggcagattcttataaagggcaaatacaggttaggaatgagcggccgaaggggaagaaaggctttaccacggcacaacttt
cttctcactcaccaactccccgtggggagaatgtgcattgttgggcaggagagctaaatacacaggggtctctgcaccttcatctggggacttggtggca
ttgggcccagccatgtcagtcctcacccaccccgggcagcaggcgttgagcagaatcccatcatctttcctcttctcattcagctccctggcttggattc
gggacagtacagtcaccccgattttggacaccccataagctgtgtttggccacccttccttctgatgggctcccttcttggcatcttctacaaacttctc
catgagggtcaccaactcctcctctgtgatggtgtcgctgcgaaacaccttctgcagttcttggctacagcgcccgagggccatgtaactcgccatgctg
gacacattaaccactcgaccacggggcttaatcagtgggaggagctcatggcaggcatctctcgtggcgaagaaattggttttcagagtcacttcggcct
gggtgccaaatggggtggtgtcggcaactttaaatgcaatgccagcgttgttaatgagcacatcgatccccccgtacttctcctttaagaaaccccccag
ggccctaatgctctgcaggtcattgatatccagctggtggaagtggggggacagcccttcctgctccttcagggcccggactgcttcttctcccaacttg
gggtccctggctgtcaggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: