XB-CLONE-3544722
Clone Name: IMAGE:7717867
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Gene:
dusp12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979197 [+]
cttgaggccctcaggcactttagcaaggactgatctgatcaagagggaacatttttactgcgtgtcatctgaaggagcttagaggcaaattcttctcagg
gattgtgttctgatttatagctatgacaagtaggttgcatttttttttcttcaagatgtaacatttcttatcggctgagggctctgggttaccaagcagg
cacctttatattactcagttctgctgatactgctcttcctcatctgcaacatgaatctgcagatatataatctactagttgggcagtttggacaaaactg
gcgaaattgccaacctaatcttggcatgaatgtccaagcttcaaatatgtctgcgcacaacaccattaaccagttttcacttgtacctcatctcttccta
aaatcaactagatctccaaagactcatatgtccttctcattctggtgaggtgtctgtagcactacatgcactctgttcaattgtcactgctgtccatggc
cctaaacctttatacttccctctatgagtacacttccaaatgaatggataaattatttcccttttcattttttgaagtatagggaagtttagggaatgtc
ccaaaatacagtttctgcactatagtaattcacttgccacaggaacactttgctttacttcattctttgcaataatgaaagggcagagagtcttgtctat
atgacaaaatatttaacatttctgatgcagatcattgtatggaacatgcctcaagagtataaaatctggcttccagcatcagcttttcacattaaacgtt
gtctgaatttagcagccacactggaacccaacaacaattg
3' EST: CX979196 [+]aaaaacagaagcaaacaattagtaattaatatcattatgaccagtggggtccatagaagatgtcttaaacctttgccgattccaaggtccactctcacgt
tggccaatcactgtttggaaagctggattcatgccatgaccaactagaaccctcccgtgtggttaaatgtaggttattattgcattctctttgtgtagct
gctgaaggattattgccatactaaaataacaaagcattgcatactgattttgtttggcaaatacaatatggagatccagctcatgttattttgcctattt
ctttctcccatatatggactgtattcctttcacatcatcttggggtagacgaaagtttccggtgttccaatagcgataggttggatacatcaaagcgttt
ggatcacgagaatgacccaaacccagcgaatgaccaaattcatgtgcggcaacaagaaacaaattatattctgctgcactatttgtccacttttcatcct
catcaaaatgagcatccccacctattccatttcctggagcaaatgcatgagccaaaacaccatgggggccatcaaaagggtcaaaatctccatgtgagcg
ttttgcaaatttgatcatgatatctgctgttcccgaagttagttttctgaaggtcagtggggcaacatcactccacagcttgaatgccctttgtatcgct
tcatctaccttttggcgagggagatcaggngtgtaattctgaattctgtatgtcagtttggtgtttttccatcgtggatgtccagggaacttgctgtatt
ctgacacatcgggcattccacatcgag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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