XB-CLONE-3544716
Clone Name: IMAGE:7717581
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Gene:
nsmce3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979201 [+]
cctacatcaagatcttcaacgttgggagccgatacctggtgaacagggttcaggatcacattcagagccgtgcagtgtactacctaatgaatatccacgt
cactccccgctccatttacctttgccggcatggagagagtgagcttaaccttttggggagaatcggtggtgattcggggctgtccacacgtggcaaacag
tttgcacacgccctgggcaactttgtgaagtcgcagcaaattaccgaccttaaggtgtggacaagtcacatgaaacgcaccatccagacggcagaagctt
taggtgttccctatgagcagtggaaggctctgaatgagatcgatgctggggtgtgtgaggaaatgacatatgaggaaatacaagagaggttcccagagga
gtttgccctaagagaccaggacaagtatcggtacagataccccaaaggagagtcctatgaggaccttgtacagaggctggaacctgtcataatggagcta
gaacggcaggagaacgtgctggtcgtctgtcatcaagcggtcatgcgctgcctgctggcctacttcctggacaagagtgcagagcaacttccgtacctga
aatgtcccctccacacagtgctgaaactaacccctgtggcatatggttgtaaagtagaatccatctatctgaatatagaggcagtgaacactcacaggga
aaagccatttaatgttgatgtttcccgtgacccagaggaagctttggacactgtgcccgaacatttttaactgtaaa
3' EST: CX979200 [+]tgttctgcaacactgatgtcttaaagtgacagttaaacttgaacgttctgttcacaataactgctggttcagctaaaatgccatatgcttgctgttgctt
ttccaagcactgcagggagtctcagctcagactacttattgtcaggaagagaagttcattttcatgttttaataaaatactatttctttgcaaaaagccc
tttgtgctgctttccttttaatttccactgagcccattcattcatattctagcagcacaactcctgctccagtgatggataaattgcaccctgcatcctc
ctaaaaaggaggtgactaaatgtaccacagggacactaacatctgactgtcctccagataccagagtttataaaattatattaaagtcactaaacatgat
taaaaataaacagacccaattactaaatatacaaacaaatcactagtgggcctgagaactaccttttagctaccttccaataaagcataactggggctct
gcctatatgggggaatagtagccacaggcaaaaaaaaaaaaaaaaaattaaccccactagccattccctccattgtacatccttactgcattcccagttt
ggcgcctcattagccactgacacttccctcatgttaaaaatcaatctgattggagcaactctaaaacaaaattaaaaatatacaaaaaactttacagtta
aaaatgttcgggcacagtgtccaaagcttcctctgggtcacgggaaacatcaacattaaatggcttttccctgtgagtgttcactgcctctatnatcaga
tagatggattctactttacaaccatatgccac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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