XB-CLONE-3544631
Clone Name: IMAGE:7717685
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Gene:
gcsh
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979261 [+]
atcagttttaattacaaggcattatttctgaacgtgcaacaaactttgtcttcagtcatggctgttggtaccaaaaaagggatggttttcattccagcac
acaaggaagaagtctttaaagaaatcactacattaccggatgttcaataaactcttcagttgctggagaactctgcagcaattacacaagaatctgaact
tattgcagcatgttaattaaggtttactaagttttagcataattattcatatctaaaaataaaaaaatgcggaattatatagtgtgcaaaagatttaggg
acaactcttgaaaaagaatcccttattccagtccttatgcaaactgcatttgattaaaaccgttactgttgcagtaagaagtccccaccagtgtgatctg
agacaccactactcaacctacacagggaaaaccttctagctgggttacaactccaaaaaattatctgatcagtcttcaagtgcctttacgtatttctcgt
aggcgtcttcactcattagatcatcaagctctgaagggttgtctactgtcagctttattagccaaccttcttcgtaacaagattgattgaccaagcctgg
attgtctgttagtgctccatttatctcagtaacttctcctgttaggggggagtaaagttcacttacagctttaacgctctctaaagtcccaaactcatcc
attttattcagctttttgccaacttctggaagactgcaatacaccacttcgcctaaagaatcctgggcaaacttgctgattcctactgttccaattccat
tttcaactgatatccattcatgtttatctgtaaatttcaggc
3' EST: CX979260 [+]ctggtatggaggtcgtgcttggtcaggagcttggccaccggctcccagttgctctcctctgccttgaaatttacagataaacatgaatggatatcagttg
aaaatggaattggaacagtaggaatcagcaagtttgcccaggattctttaggcgaagtggtgtattgcagtcttccagaagttggcaaaaagctgaataa
aatggatgagtttgggactttagagagcgttaaagctgtaagtgaactttactcccccctaacaggagaagttactgagataaatggagcactaacagac
aatccaggcttggtcaatcaatcttgttacgaagaaggttggctaataaagctgacagtagacaacccttcagagcttgatgatctaatgagtgaagacg
cctacgagaaatacgtaaaggcacttgaagactgatcagataattttttggagttgtaacccagctagaaggttttccctgtgtaggttgagtagtggtg
tctcagatcacactggtggggacttcttactgcaacagtaacggttttaatcaaatgcagtttgcataaggactggaataagggattctttttcaagagt
tgtccctaaatcttttgcacactatataattccgcatttttttatttttagatatgaataattatgctaaaacttagtaaaccttaattaacatgctgca
ataagttcagattcttgtgtaattgctgcagagttctccagcaactgaagagtttattgaacatccggtaatgtagtgatttctttaaagacttcttcct
tgtgtgctggaatgaaaaccatcccttttttggtaccacagccatgactgaagacaaagtttgttgcacgttcagaaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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