XB-CLONE-3544595
Clone Name: IMAGE:7717545
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Gene:
adss2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979286 [+]
cggagtatgctgcgccactgacagtggacgttgctccctcgtcgggaacaaagtcacagttgtgcttggggctcagtggggagacgaagggaagggtaaa
gttgtggatcttctggctcaggatgcggatatcgtgtgccgctgccagggaggaaacaatgcaggccacacggttgttgtggattcagttgaatatgatt
ttcaccttttgccaagtggaatcattaatcaaaatgctattgctttcataggaaatggtgtagtaattcatctacctggcttatttgaagaagcagagaa
aaatttaaagaaagggcaaggtttggttggctgggagaagagattgtgtatctctgatagagctcacattgtgtttgacttccatcaggctgcagatggc
atccaggagcaacaacggcaagaacaagctgggaaaaatttgggcacaacaaagaagggtattggaccagtctattcttcaaaagctgctaggagtgggc
tcagaatgtgtgatcttgtttctgacttcagtgaattctctcagaggtttaaattgttggctaagcagtacaagtccatgtacccatccttagaaataga
cattgatggtgaattaaaaaagcttcaggattatgctgacagagtaaaaccgatggttaaagatggtgtttactatctatatgaagcccttcatggtcca
cctaagaacattctagtagaaggtgcaaatgctgctcttttggatattgattttggaacatatccttttgtgacatcttcaaactgtactgttnggggag
tatgtacgggc
3' EST: CX979285 [+]tttatgcaaccatgcttttcttgggttggaaccttaaactcttattcaataatttacaaatatagactgtctacccatttgttttgcacagtgcaagtat
tgcagtgcaatacattttaaaacaaaatcattcacaccaattatgattgctttaaaaatatggatatttcctccccatacattacatgagttatgttaag
aaatcaatagaaagatgtaaaaatgtgtaaaagaattctgattcagtctcacaacttgaaattgcagactgtcaacaaagttgtaccagatgcgcacata
taggtactagggtgttttttttaattttatacagaaaaaatagccagttcaatgtgtgatggtcctttccaaaactgaacaaagaagtcactcagtcttt
aaagtactgccagtttaataaccagaccacaaatgtcctaatgcgatagaaaataaacagtaggacaattttgactgctttcttttactagattgtgaaa
aagactaaataaattagggcataacaaaagatgaatctggaagtgatattttgttgcccatgcatttgttctctatgtacaagtgagaagctttatctgt
agaatggtgtcatcttgaggaggcagcctgtgcagtgtgcatcttcttagaacagctgaatcatcgactctctggattttccaacaccaatccatttaat
aggaattccaagttctaattctatatatcgaacatatttctttgcattttcaggcagctcttcaaatgttcgaacgttacatgtatctgtattccaccca
ggaagtgtctcatattcaacctctact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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