XB-CLONE-3544582
Clone Name: IMAGE:7717595
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Gene:
etnppl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979296 [+]
cagcagcagagatgtgtgaggaggagtactgcaaggaaaagatcattgagatgagaaaaaagcacattgggccttcgtgcaaagtgttttttgcaaatga
ccctatcaagattgtgcgtgcaaaaggacagtacatgtttgatgaaaaaggagagaaatacttggactgcataaataatgttgctcatgttggtcatagt
cacccagaagtaaccaaggctgctttgaaacagatggaattgttaaacaccaattctaggtttctacatgacaaccttgtccggtatgctgagtgtctta
cagccacccttcctgagaagctttcagtctgctattttgtcaactcaggctcagaagccaatgaccttgcactgcgtttggcacgacagtacagtggcca
tcaggatgtcataactcttgataatgcttaccatggccatgtaacctccctaattgatattagtccctataaattccaacagctgggaaaggatgcacag
aaggagtttgtacatgtggctccttctcctgatacttacagaggaaagtacagggaagatcatacagaccctgcattagcttatgctaaagacgtagagg
acatcattaaggaggttcataagaacaagcgtcagattgctgcttttattgctgagtccatgcagagttgtggaggtcaaataatcccaccagcaggcta
cttccagaaagtgtcagagtttgtccataaagcagggggagtcttcatagcagatgaagttcaggtcg
3' EST: CX979295 [+]cttaaaatagctttattttcagcaagtcattcacaaaaatggtcagaattcattcatttgtataggttgtcatcctgcagcactgagcaaggaagaaaat
gtatggaaggaggtatgaggggaaagtatatactgtaagggtataaaacttaccctttagcattaaggccttttgggaacactttgggacaaactgtgga
actgccatacagattacacagacagaggaaatctctaacagaataagtggttatttatagtcactttccctacacttcaacacggctttaggaccgatgt
ataaatatacagaaagactgaatgtcctccagtcggacatgctatgttttcatactttataggcacttttagggagattaatccagtcacggggcagaga
tttccccaaactaggataggaacagtagtgttatttgcaaatgcacagcaaccatccaaccggcaagcattctgcaagcctctagtgtccaatgaggtga
gagtcagttgcaattatatataggatttagcctgtgcttatacaattacctattagacctttggggtgttagccagagggaagtacagctttaaacccac
tctcgattacaaactttgaattatttgctttttttttttattctccctctttccagtcctcaatttaatgatggcatgtggttgccaggataagtcttcc
tagcatccaaataattgacttatgcagaggatttgattatattttacattatatcctgctcttaaaaggtctctcattgcaatatgcttttacatttgct
ctggtgttggttggtatgactagtgtccccaacaacttggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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