XB-CLONE-3544576
Clone Name: IMAGE:7717691
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Gene:
nfkb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979300 [+]
atccaatccatgttgctgtactttcaaattgtgtgctgtgcctccggctactgatatcagccagggcagatgtaaacgctcaagaacagaaatcaggaaa
aagtgcattgcatttggcggtggaacaagacaacatatctctagctggctgtcttcttctggagggagatgcgtgtgttgacaatactacatatgatggc
aatacaccgcttcacatagcagttggaagaggctctaccaagcttacagcacttctcaaagccgcaggtgcagattcctttgttgagaactttgaacccc
tcttaagatttgaagatactcaagatgaagatgatgtagatgaaggaattgtaccgggcacaaaacctctggacatagctactagcgatgaggttcttga
aattctgaatggcaagccttacaagtcagaagtggctcctgaagactccttgccacaaggtgacatgaaaaaccttagtggagaggtaaagcagaggctg
tacaccttgttagaaagccaagatttggacaagaactgggtcactttagcacagaagctgggccttggaatgctgaacaatgctttcagattaagccccc
acccatctaaaacattactggacaactttgaggtttctggtggaaccatgacagaactcctggatgccttaaaactgatgcattacactgaagctgttga
ggccattgagcagtcacaagctatccttaagctgtctgggacttctacgccatcttcagaggcactgcttcctgcacag
3' EST: CX979299 [+]cagaaaaatatataatatttactgtagtaaaccaaggcataaaacattattaaatgattgctagccattcttaatatattgccaacatttgcaaatgaca
gtaacttaactgggaagtttaatttctgaattgttttttgaatcgtgtagttttctgatggtgtataataggaactcatgcagaatccatagagacttta
aaccaacatgattcaataagtaaatatgcaacatcctaattcaattagggataagcaatacacagagtgtgatgcccagaacccagaagcttaggtcctt
aaaagttgcatatatatataattaagaacccagaagcttaggtccttaaaagttgcatatatatataattatatatattcttttcgctagcttactatat
gcatgagcaaagaagtgttttaaattgtaccaggcccaggctttcttgaatatccagtgtatttgttcaagctgctgagcctcgctgtgcatgtaagaca
gagtccaacacttgcctatcacttgttgcagatgagagtacagtctggttcctgggaaaactttgggcaaactgtctctgtctccttcagagattccgaa
tatgaaaggctcagtttctgaaaggatgtctctacgccactgtcgcacgtactatcagtgtcctgcagcttaaggtaaccaacatcgaacatgggggaat
ctcttctctgtgcaggaagcagtgcctctgaagatggcgtagaagtcccagacagcttaaggatagcttgtgactgctcaatggcctcaacagcttcagt
gtaatgcatcagttttaaggcatccaggagttctgtcat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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