XB-CLONE-3544500
Clone Name: IMAGE:7717889
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Gene:
hsp90ab1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979353 [+]
aaaagatctttattttgcgcaacatttctcaactttacaacaacaaaaaataaaataaagttccgtattgaggctctcgatagagagatgtgtaggatgg
gggtggggtctcagcattcaagacatcctgggggcgggggcacgagattccagtcagagagatgggggctaccactcagcagatttgggggtgctacaga
tggaggtagctttgggcagaagtacctcacccccccagactggcctggcgtttctatttgcaaacaccagcacattcaatcgcccccccccatgcaagtg
aatgggagaaggcagcaggggggtctctaaagcttcccagtaagtaccccccgtgcctcattaacctatcaggcacagagaggggcacgcccattacaat
agtggcagagggtaggattagaggggggcctgtaaggtgcattgagagagtcgaacagacccccccagcagctggctaacagcgggccactaactcaggg
ctaccacaagccaagctagctgccatactgctacaataacttacataactaactgctcagactggaacacaacaaggggggtgcagaggaaagggttaaa
gtcagcagccatcagtcttgaagctcccccaaatatattctataaggagggaagtccatccccagtatcatgtgatatttagagtccaagggagaagtcg
cccccctaccgagttacagacagtgcacatacgcgggtgggagggggcaggacgagcgggcagcaaaccgattagtcgactt
3' EST: CX979352 [+]acggagagttctacaagagcctgaccaatgactgggaggatcacttggctgtgaagcacttctctgtggaggggcaactggagttccgcgctctgctctt
cattccccgccgcgccccctttgatctgtttgagaacaagaagaagaagaacaacatcaagctgtacgtgcgccgcgtcttcatcatggatagctgcgac
gagctgatccccgagtatctgaacttcattcgcggggtggtggattctgaggatctgcccttgaacatttcccgtgagatgctgcagcagagcaagatcc
tgaaggtgatccgcaagaacattgtcaagaagtgcctggagctcttcaccgagctggccgaggacaaggagaactacaagaagttttacgaggctttctc
caagaacctgaagctcggcatccacgaggattctaccaaccgcaagaagctgtctgagctcctcaggtaccacacttcccagacaggcgatgagatggcc
tccctaacagagtacgtgtcccgtatgaaggagaaccagaaaagcatctactacataactggtgagagcaaggaccaggtggccaactccgccttcgtgg
agcgggtgaggaagcgtggcttcgaggtggtctacatgacggagcccatcgacgagtactgcgtgcagcagctgaaggagttcgacgggaagacgctggt
gtctgtgaccaaggagggcctggagctgccagaggacgaggaagagaagaagatgatggaagagaacaaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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