XB-CLONE-3544270
Clone Name: IMAGE:7717816
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Gene:
LOC101733035
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC155466 [+]
TAAGTGTCTCTACTGCTCATACAGTGGTCCATAGAGTTGGGGTATACCGCCATGGGATCTACCCCTCGTACCTATAGACTTATAATGTCATTATTATCTT
GACTCCAGAAGGTGAATGATTAGAGAAACTGTCATTTTTTGCTACAATGTCCTCACTTCATCCTGATAGTTCTTCCCTCACAAGAAATGTTTGTAGGTAC
AGTATCTAACCTACCAAGATATCTTAGACAATGAACTGCCTATCTATGATTTTCAATATATACTTATATAATATCAGGACTTGATTGGATGTGCTGCTAT
TTGTTTTTGTTTATTTTTAGGGATCTACGGATTTGCCAAGGAAGATGCAAAGCTATCTAAGGCCTGTGGGAAACCTGTGGTGGACAGGAGCCGCATTGTG
GGAGGGCAGGATTCAAAGGAGGGGCAGCATCCCTGGCAAGTGATTGTGATGCTTCCTAAAGTCCAATGTGGTGGAACCCTAATTAGTAGCAGATTTGTTC
TGACTGCAGCCTCGTGTGTGCACAGTGTTAAAGACGTCTTGTTTCACGTTGTGCTTGGGGCCTACAACTTAACCGAATGGACAGGATTTTATGTTGCAGT
GAGTCGTACCATCATACATGATAGATATATTTACCCCAATTACTATGATGACATTGCCCTTCTGGAGCTTTCGGTGAGCGTTCCCTTCACGGGCCTTATG
CTTCCGGCCTGTCTGCCTACGGCTTCCACCCAATTTCTGCCCGGGCACAGCTGCATAATGACTGGCTGGGGAGACACTGATTATGATAATTCCAACCAAA
TGCAACTTATTCTGCAAGAAGCGGAAGTGCAGTTGATAAGTTTAGAGCGTTGCAGAGACTTGTATAGTCAAACAAACATTCTCATTGCGGATACTATGAC
GTGTGCAATGGACATCCATGAAAAAGCAGGAATCTGCGCTGGTGATCTTGGAGGACCCCTGGTTTGTCAAAAGGGGGGGCAGTGGTTCCTGGTTGGAGTG
GTGAGCCTTGGACTTAATTGTGTAGCTGCCCTTCCTGTAGTTTACACCTCTGTGCCTGTCTATATGGATTGGATTAATAAACACGTTTTCCCACCCAATG
GTGGCTCAGGATTGATTGGATCAATGCCTGGGGTGGCACTTGAGGTCCAGAGCTTGTCTGCCTTTATCTTCTCTCTCCGTTCTCTGTTTTGGGTGAGCCT
GCAGAACCCCATGTTTATTTTGATGCTTAGCACTGTTATCTGGGTGGGGTGCTGAGCCGTGACTGCAGCGGATCATTGGAGGAATGTTCTACAAGAACCT
CAAAGAGGTGGAACCGCAATTGCATTCTCTGGATTATTTTCTATTAAAACTACTTACTGAATTATGAAAAAAAAAAAAAAAAAA
5' EST: CX979508 [+]taagtgtctctactgctcatacagtggtccatagagttggggtataccgccatgggatctacccctcgtacctatagacttataatgtcattattatctt
gactccagaaggtgaatgattagagaaactgtcattttttgctacaatgtcctcacttcatcctgatagttcttccctcacaagaaatgtttgtaggtac
agtatctaacctaccaagatatcttagacaatgaactgcctatctatgattttcaatatatacttatataatatcaggacttgattggatgtgctgctat
ttgtttttgtttatttttagggatctacggatttgccaaggaagatgcaaagctatctaaggcctgtgggaaacctgtggtggacaggagccgcattgtg
ggagggcaggattcaaaggaggggcagcatccctggcaagtgattgtgatgcttcctaaagtccaatgtggtggaaccctaattagtagcagatttgttc
tgactgcagcctcgtgtgtgcacagtgttaaagacgtcttgtttcacgttgtgcttggggcctacaacttaaccgaatggacaggattttatgttgcagt
gagtcgtaccatcatacatgatagatatatttaccccaattactatgatgacattgcccttctggagctttcggtgagcgttcccttcacgggccttatg
cttccggcctgtctgcctacggcttccacccaatttctgcccgggcacagctgcataatgactggctggggagacactgattatgataattcccaacaaa
tgcaacttattctgca
3' EST: CX979507 [+]ctaattcagtaagtagttttaatagaaaataatccagagaatgcaattgcggttccacctctttgaggttcttgtagaacattcctccaatgatccgctg
cagtcacggctcagcaccccacccagataacagtgctaagcatcaaaataaacatggggttctgcaggctcacccaaaacagagaacggagagagaagat
aaaggcagacaagctctggacctcaagtgccaccccaggcattgatccaatcaatcctgagccaccattgggtgggaaaacgtgtttattaatccaatcc
atatagacaggcacagaggtgtaaactacaggaagggcagctacacaattaagtccaaggctcaccactccaaccaggaaccactgcccccccttttgac
aaaccaggggtcctccaagatcaccagcgcagattcctgctttttcatggatgtccattgcacacgtcatagtatccgcaatgagaatgtttgtttgact
atacaagtctctgcaacgctctaaacttatcaactgcacttccgcttcttgcagaataagttgcatttggttggaattatcataatcagtgtctccccag
ccagtcattatgcagctgtgcccgggcagaaattgggtggaagccgtaggcagacaggccggaagcataaggcccgtgaagggaacgctcaccgaaagct
ccagaagggcaatgtcatcatagtaattggggtaaatatatctatcatgtatgatggtacgactcactgcaacataaaatcctgtccattcggttaagtt
gtaggccccaagcacaacgtgaaacaagacgtctttaacactgtgcaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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