XB-CLONE-3544201
Clone Name: IMAGE:7717680
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Gene:
kdelr1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979556 [+]
caggatcctaaaaatggaaaatgaccaaagaaaataagtgaatggcgtaggacagattttggcattaatcagtcagtgcctgaaaggggcgtcgtcatca
tcacgattatcgagctggtcggctgtcttgcgtcagtgaaaacagcagttccatgtcacatcccgagcaagttaagaatgacaaaaatccgagtttttct
ttatgaaagatactttagtacaattttccttttacaacactttgaataaaaaaatatcacctcaccttctcgtgtggcccacatacgttatcccttatgc
taccacagccttgattactcaccaggtgcgggaatttgaagtgttaataaaaacggaaccgttttggtgttttagtgaaatgtgaaggtatatcaaaata
caggtttgtattggaccaatggcaatgcattccatctgctgccagagagcctcacaacaaatcactgttaatgcgtcatgatcaatatttatttggaaga
aaaaaaaccttttttgaatatttgtatctttaaaaagaactaaagaaattaaaaaaaaaaaaaaaaaaagaatcccccctcccaccccatgcgcctccca
tagagaatgtagaaaaaaaaaacctgttgaaacatcacaaaaccagaatatgtatcgtctaaaacaaaataaattatagtggaatgactggagttaaatg
gatacaatccagtaaaggggctcaaacgccccttaatgagctcggaacagagaggggaagaaagaattttgcacttgaacctagttgtaaactgcac
3' EST: CX979555 [+]tgcactggtgtttaccacccggtacctggatctcttgacatcatttatttcattgtacaacacatctatgaaggtaatctatatcggatgtgcatatgca
acagtctatctgatatacatgaaattcaaggctacatatgatgggaaccatgacactttccgagtggagtttcttgtagtccctgttggtggattatcag
tcctggtcaatcacgacttttctcctcttgagatcctgtggacattttccatctacctggagtctgttgcaatcctgcctcagttgttcatgatcagtaa
aacaggagaagcagagacaatcaccacccattacctgttcttcctgggcttgtaccgtgccttgtacctcttcaactggatttggcgttattcttttgag
ggcttctttgatctgattgccattgtggcaggtgtggtgcagactatcctttattgtgacttcttctacttgtatgtgacaaaagtgcttaaaggaaaga
aactaagtttgccagcgtaagtgccaaagattatgaccagcagctgtcccctccaccggctggacagaacaatccttacttcgtgtgaaggtgtaagatg
cctgaagcagagtcagaaacactacttttatataccgaactgccctgtttgctgccggagaggcacagaagcaggtcccgggccagcccctccctcagca
aattcgccaagtcaacgacgactcgattaacaacctgatttgttttatgcatcttgcctttcctggttttatattatgatgtataaagattttttttaca
taagaaacgtatgctgtaatgagaaatttcagtgcgca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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