XB-CLONE-3544067
Clone Name: IMAGE:7717836
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Gene:
ciao2b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979648 [+]
ccagttcctatcattattatactcgatccatttagggtgaaacattaatttcccagtcctgttctggaaatagtggtgcgtatgccccacccacgatata
tattatatacacattataacagcctgctctgtattaatgatgtgtggacctcccaaaacccgtgggggtcggcacttgcacaacatattcaggttggggg
cgggtttaagctgagctattcccacccacgacctaacggtaccccctctgccccacccccttcatgacatcagagatggggaaggcggggtctatgtaaa
cagacgctgatttgcagcctagttggaaatgggtttgtggggcgggggccggttatctgttggcctacacatcactaatccacccacacagagcagaaca
tgactgggacattacagtgacaccctgaatggactgagtctctgcccaggcccggactggcaatctgtgggttctggccaatgccagaggggctgcagtc
actatttattgggccggtgggggggctgtttgggcctcggtgtacttgatatgtcagggtctattctaaatcccagtccgggcctgactttgccttttcc
tgtttaaaggtcacatgtagcggcaggagtgcatgctgggtacccataaccccccagcagaacggctgatttaagcagaggatctgccggataagcattg
gttgacgacttccagcaaatgggaattctctagggcggcggccacgcgctccttgtcggccactgcttgttcactgcgtgctcagaagcgtgcgtgccgg
gagttatatggacgtcca
3' EST: CX979647 [+]cgcgaatccgctgatttaccggcgggcgggggagcggcaggtcacagcgcaggaggaggatgaggacgcgccggaccgaatcgatgaccgggagatcttt
gatctgattcgctgtataaatgacccggaacacccacttacgctggaggaactaaacgtggttgaagaaattcgagtgaaggtaagtgatgaggagagca
cagtgtccgtcgagtttaccccgaccatcccgcactgcagtatggcaacgctcatcggcctctccattaaagtaaagctcctgcgctctctgcccgagag
gtttaaggtggacgtccatataactcccggcacgcacgcctctgagcacgcagtgaacaagcagttggccgacaaggagcgcgtggccgccgccctagag
aattcccatttgctggaagtcgtcaaccaatgcttatccggcagatcctctgcttaaatcagccgttctgctggggggttatgggtacccagcatgcact
cctgccgctacatgtgacctttaaacaggaaaaggcaaagtcaggcccggactgggatttagaatagaccctgacatatcaagtacaccgaggcccaaac
agcccccccaccggcccaataaatagtgactgcagcccctctggcattggccagaacccacagattgccagtccgggcctgggcagagactcagtccatt
cagggtgtcactgtaatgtcccagtcatgttctgctctgtgtgggtggattagtgatgtgtaggccaacagataaccggcccccgccccacaaacccatt
tccaactaggctgcaaatcagcgtctgtttacatag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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