XB-CLONE-3544008
Clone Name: IMAGE:7717794
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Gene:
atp1b1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979688 [+]
tttattgtctcatgtttaacttgactgaagttccaacaagagcacaggactcctggcaattattacagatgtatgaacaatgtgtgcacagtagacattt
aaatacatcctatgggatactgcattgccatgaacgttgttttaaaataaagatttatttttcaacaggttactttggtttgtttcatacattttctttt
tcttgaagaaaaataaaaattgtacaactgcataaatataaaattcttccaccatgaaaatggttaaaacatttgtaaagacaaagcaccaaactgtgat
gctttcagaaaaaacaaaaaaacaaaaacataaaataaaaatatttatatatatataactcaaaaaccattagccctatttctgacaaaaagtgacagtc
ggacccggggcttcacacaccttcataatgggcttaagcatttaaaggggaaagtttctataaaaagcattaaataaaaagcaccgcacaacccatcacc
tcttgcatttcagtgaaactaagaagagaaagacaaaaacagaagctcttttacagataaaacaaatcaatgccttatttaaaggacttgtggagtgcta
ttaaatattattggttggccaccacttccgtttaaattaaaaaaagaaaaaacaaaacaagaaaagtagcaataaaattaccatgccagttttatcccca
ttacagactatttacaccttggactacaaacttgctcacaaagcagaaacaaaacaaaacatggcttggccgagactgtagaatctgcacttatcctact
gtacaaa
3' EST: CX979687 [+]ccgctgccgatcggctttcccatacccgcatacagtaaccagcgtgacagtggccagccagtcccattgttagataacaggagaattgtagggacaagga
agaagaagcagcccagtcacgcttcccatcagcgcccagtgacatctcaatagccggtaccgcaaaataacacagaacccttaactgaagtaggacaata
tggccagggacaaagccaaggaaaccgacggcggctggaggaaatttatctggaatcccgataagaaggaatttcttggcagaacaggtggcagctggtt
taaaattcttctgttctatctcatcttctatgggtgccttgctggcatatttattgggaccattcaagtactcctgttaaccatcagtgaatatgaacca
aagtatcaagatcgtgttgcacctccaggactgacacaagtacccaaagccgtcaaaactgaaatcaacttttcaccaaacgatcctgacagctacaatg
attacgtgcaaagcatggaaaagtttatttcgaagtacagcaacgagaaccaagtatccgataagtttgaagattgtggcactatgcctgggcagtaccg
tgagagggggggtcttaacaaggatgggggacagaaaaaatcctgtgtgttcagacgtcaatggttgcagaactgctctggcatagatgatcaaacattt
ggatttgcagagggcaagccatgcgtcatcgtgaagctcaaccgaattgtggcatttaagccagtgcctccacagaataacagtcttccaccagaaatga
cagcgaactacaacccatacgtcatccctatccactgtcaggggcagagagatgaagacatacctaacattcgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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