XB-CLONE-3543984
Clone Name: IMAGE:7717796
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Gene:
btrc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979704 [+]
gaatacatatacactaaatgggatcatgggaacctgcattggaaagcatggctccctacccataggccaacaatagccaatataaaggaatttaatagga
gcacagacaccaatgtcaaacaatttctttacatgatcacaatcctaaacactaactgaattataaaaaaaaaaaaaaatctatgcactgtggtgatttc
gagagcagatttttacagtgtctctttaaaaaaaacaaacaaacccccacaagtttggctttaagtaaaggacagaaaacgagtgcttttttttttcttc
ttctaaaggacaaaaagatactttcctgttgcgttacgttaagtgtttgtacctcagttttcagatcatgcagaatatttaatagtggttcacatgcaag
aagagatgagtttaaatacgtatttacgaagagcgctatggcaaatagggctgcccgcgaagatcagaagcatgagttagtagtcgtgtgcagaaaatat
acatggtgttttgtgacggtcagtacaagcgcggtgacggaaataaccaccaagaaagacgaacgtactgctgtgccaagccaagggaagagggagaaag
aagaggagaaccctcactagaaaaataagaggagacacttaaatggagacacataccaagagggaaagtgagttttgcgctatccttacccttgtgccat
gtgataagaacatctctaatcagtttattttttttctaagcttttaatggcccataaagaactatacagataactacaacacttgatttggtactatatg
caaaaaaaaaaaaggaa
3' EST: CX979703 [+]ctcatctgggattttctcaacgacccagctgcacatgcagagtcaacgcgttccccttctagaacatacacgtacatctccagataaatgacactacgta
gtacctcagagttgcacaggacttgcttgagaagtatggtaactaaaccacctgtgaacgccgggaaataaacattctacttgaccagtatttgctgaaa
aaaaaaaaatatagagagagagacccattatcctgactcatcctggaatattgctgtcaggcagctggaagcagcccgggccaatctgcacagtggactg
catgattgctgccatgagaagaattcttgtatctccgtcattgtcaccggagtctgcaacttattgcgtggcggtgggttgcacactccagtggcggcgg
ctcattactcaaaacttgcaatttagagatgaaacctgagagactgtagcagtcacagatctttgaaatctatttaaatgtcttgccaggattcctgttg
cttcgctgtcagcaaaagaacaattttcatttttgtcccacgctcacgctgctttaaggtgtggttccattcggagatctgctgaacgttttgcaagtac
ttgtgaagtgaaaagcgggaaatgggttactttcttttaagatgatgactaagacccccccaagtgatataaaaatgtatgtttttatcatagtaagcat
gaaaggttattttttatttattattttttttttccattaattttttttttcattcttcagtgtttgcgtgtatatccagtttttcttttttttccttttt
ttttttttgcatatag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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