XB-CLONE-3543969
Clone Name: IMAGE:7717654
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Gene:
arhgap27
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979714 [+]
aaacggctctgagtatctcatacaccatgattcagagacgatcactgcagactggcacgaatcaataaagaacagcattgccaggaatatacgtgtatct
gcagatcatacgcctgaagatgagcctgaaccattccaggaatttggttctactgagaagttgggcacaaaagatgaaaaaaagaactcaagcatgggat
caggcagcaactctgacagcgatcgcaaagtccgcgctaagctcaagaagtttttacaaaggcggccgacgatacagtccctgcgagacagaggttacat
taaagaacaagtgtttggatgccccttgcaacagctctgtgaaagagagaaacagaatgttccggactttgtaaggaaggccatccaagccgtggaaaaa
agaggacttgatattgatggtttatatcgagtgagcggaaatctggccactatacagaaactacgccacaaagtggatcaagaggaaaacaccaacctgg
aagacgggcgctgggaagacgtccatgttatcaccggagccctgaagcttttcttccgggagctgccagaacctctgttcccattcagtcactttgacat
gttcattgagacaataaaattgaatgacccggcgctgaagaagaaacagttcaaggagctgatacagtcgctgcctccgcccaatcaggagaccatgcag
tttctcttccgacatctatgcaaagtcattgaatgtaaagattccaacagaatgtccattcagagtgtcgccattgtgtttgggccgaccctcctaaggc
cctcgatagaaggggccaacatcgccatgtacat
3' EST: CX979713 [+]ctttcttgaaaaacattttaatgtaaaaagtgcaacagggaaaagagatgaatcccaggctgtttctctctgtgtagcagcggggcccctggctgggctt
gcaccgcgttaaatagaatctccattggcattgcagacttctgccaaatatatatatttatatataaatctgtgcttgaaaataagagaaacctacagca
gagtcggatagaaaacaatctatagtggctgcttcattaaagtgaatattgacattttcagtggggagatttatttacattaacaatgagcaagtcaccc
ccctttccctactatagcttattttgtacatacatacacatatatatatctatatataactcatgagcacaagtatttacacaagaacatttaaatagcg
aggcagaacaatataaaggctttctcgggcgggcccgcgccgtcatgtcacagtcggggggaatccacgtagcaagggatcagcttgtgttaaatatgta
tttgtattggcttagaacttgctccacgatttggttctgaaacaccatgtacatggcgatgttggccccttctatcgagggccttaggagggtcggccca
aacacaatggcgacactctgaatggacattctgttggaatctttacattcaatgactttgcatagatgtcggaagagaaactgcatggtctcctgattgg
gcggaggcagcgactgtatcagctccttgaactgtttcttcttcagcgccgggtcattcaattttattgtctcaatgaacatgtcaaagtgactgaatgg
gaacagaagttctggcag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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