XB-CLONE-3543947
Clone Name: IMAGE:7717656
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979730 [+]
cataacatgcgtgttatgaagtttagtgtcagccctgttgtgcgtgttgctgtggaagcaaagaatcctgctgatctccctaagttggttgagggtctga
aacgtctagctaaatcagaccccatggttcagtgcatcattgaagaatctggcgagcatatcattgctggagctggggagctgcatttggagatttgcct
gaaggatcttgaggaggatcatgcctgcattccaataaagaaatctgatccagtcgtatcttaccgtgagactgtgagcgaggagtccaaccagatgtgc
ctgtcaaagtctcctaacaagcacaaccgtctgtttatgaagtgtcgaccttttcctgatggtcttgctgaagacattgataaaggtgatgtatctgctc
gccaggagttgaagactcgtgcccgctacttggctgaaaagtacgaatgggatgtgactgaagctaggaagatttggtgttttggtcctgatggctctgg
gcccaacatacttactgatgttactaagggagttcagtatctgaatgaaatcaaagacagcgttgttgcaggattccagtgggctacaaaggagggtgtc
ctgtgtgaagaaaatttgagaggtgttcgttttgatgtccatgatgtaacacttcatgctgatgccatccatagaggaggaggacagatcattccaactg
cacgccgcgtcttgtatgcttgtgtgttgactgctcagccacgtctgatggaaccaatctaccttgttgaaattcagtgccctgagcaggtggtaggagg
tatctatggtgtcttg
3' EST: CX979729 [+]cctatagggcgagaggttgcagtacatctttgctctacattctgccctcgttcctgtcaaccctcaaattcacatttacttccccagccatccaagatgt
tttcagagagcagaagctacccatcaagaataaatagaaagttatgatcatgccaatgaaagattatattcagggttccaacagaatgttgcagggaatg
ctgacatcttttgggtatagttcatttccacttaatcaatgctgacaggagtaaagcaaaggtttacagcttgtcaaggaagttatccaaggcttgaaca
ccctctttcaagcccttcctcttccgggtctctgccaccacttggaaaggcctggtggagttatcgaaaggatctccaggtagaatttgccagtggtcaa
acacacactgagggaaggcctggccaccagtgtttgatcttaagtcagcagtgaaaccaaatgactcatttacaggcaggtatgccttaaccacaaacat
tggtgttccagccacctgggattcttcaaagacatgcccacgctttctgttcaagacaccatagatacctcctaccacctgctcagggcactgaatttca
acaaggtagattggttccatcagacgtggctgagcagtcaacacacaagcatacaagacgcggcgtgcagttggaatgatctgtcctcctcctctatgga
tggcatcagcatgaagtgttacatcatggacatcaaaacgaacacctctcaaattttcttcacacaggacaccctcctttgtagcccactggaatcctgc
aacaacgctgtctttgatttcattcagatactgaactcccttagtaacatcagtaagtatgttgggcccagaggcatca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: