XB-CLONE-3543883
Clone Name: IMAGE:7717901
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Gene:
ccnk
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979772 [+]
aaaaatgtgtagggtcacagttagatattgccagccactctaagcgtgttacaggaagcactgaagagcactacttaaggcacaacatttgttaacaaaa
gggatatgctgttacatttctgctttccattaaaacctatttacaagcatttgttaaagtcagtattacaaagaacaaaacaaagaaaacaagaaaaagt
cacttggtttcagtttttttttttttttaccgactagaaccaacatctgaaatctcaaacatatatatacatttatctcagccaatatggcttcaaaata
ttctcaacagggagcagtccatagtattcaaagcataaacaaaatgcaaaaaaaaaaaacccaaaaaacctgctttccaagtccttgcctgcttcccctc
tgtggttgtaaagtggaattcctgctctaggtctcagtagcaagggggcataaaggtggtggctaaagcaagtgccataggcaggaatacaaatgttgaa
agcagtttcaagatagcatctgaagctgcaagagtccccataatcagccttttactaaaatccccctatgatgttgctagagacacttctggagtccgtt
atatacattcttacaatatgctggaaagtatcttgtttttgaacagttgaaggggagagattataaagacaaaaatatactacttcaccctttggaaata
aggaacttcatttcttaaacaatgaaatgaatctctaaatgcagcttgcacatggacctcccaccagtggtcttctctccctttgcctacatgtgtggga
cagattgct
3' EST: CX979771 [+]atttgccctatcatcctcatgtctttccacctaatcctccacccactgtaccaccgccaccaccaccttcttcattcccacctcctaacattcctcctcc
tacacctgtttatccaccacctccagcatacaaccccaattttcctcctcctagactgccacccactcatacagttcccagtcatccccctccaggaatt
gccatgccaccaacctcctatcctcctcctgctgtcccaccgggtgggcagcctcctgttgtacctcctccaattccaccacctgggatgccaccagttg
cagggctgggccgtgctgcttggatgagataaccatgctttcttgctatcatatctaagcttttcactgcagggggagagttttaatccctgattacatt
tttttattgcccatcataaaaacactggttaataccaattaaaaaagcagctttctaaaactagaagagactgaagtttctataatttactgtctactgc
aagcagatgtttaatcctttgaaatagtgttagtctcttttctttagcttgttgaaatgtgtacaaggatgcaaaatgaaaatacataatgtaagcttca
tattatggaaataaggaaggcttcttaatcttatcaattagatgttctaatataaatttactcttcagttcttcagtgcccccccttacctcccagcaat
ctgtcccacacatgtaggcaaagggagagaagaccactggtgggaggtccatgtgcaagctgcatttagagattcatttcattgtttaagaaatgaagtt
ccttatttccaaagggtgaagtagtatatttttgtctttaaatctctccccttcaactgtcaaaaac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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