XB-CLONE-3543849
Clone Name: IMAGE:7718095
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Gene:
kyat1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979794 [+]
taatgtttagcagcgtcatggccgctccttcctgagactcagcaacacagacctgagggaaactaacacctgaacatgaaaacattgcttcatgcatttc
agaggtcctatatccacaggtgggggggtcatcggctgatccccagaacagacttcggcacttgtcagcctgagaaaaggggctcagcagtcaggagcat
ctcttgttctgtgcaggacgccatggcaaagcagttgcaggcccggaggctgaagggagtggatgagaatatctgggtagacctggtcagtttggccgct
cagtacaaaccagtgaatctggggcagggattcccagacttccccccgccagcgtttgtgagagaagcctttgccagggccgttactgaggagccactgc
tgaaccaatacacacgagcctttggacaccccctcctggtgcagaaccttgcccgactctttggccagctgctagggcaggacctagatcctttctctaa
tgtcttggtgtcagtgggtgcatacggggccctattctgtgcattccaggcttttgtggatgaaggtgatgaggttattatcattgagccattttttgac
tgttatgagcccatgaccaagatggccggagggaagtgcgtgtttatcccactcagacctaaacccgcagaggctggagtgcccatgactagtgggagtt
ggagactggacctggaagagctggctgggaaaatcagccgtcacactaaagcccttgttataaacactcctaataaccccctggggaaggtctttaggaa
agaggagctgga
3' EST: CX979793 [+]tgatctgccgacccatttctcagttctttaatatgttctgtgcagcctccagagtgctgtgctcctttacaaagcagaacctgaggaagttctcaaagta
cttcttgtgcggtgcgctgaagaatgccgacacgggtatggccgctaagtgcttattcttcatcatccacttggcaaagtagtaatcataaggtatgttg
gcatctgaaggagggggcacctcagtcctgaaatgggaaatatcggcgatcataaagtaggatccctgcggcattatcggctccatccccacctccctca
ggcagtggtacaggcggtctcgcttcttctccagttcatctcgcaactgcacaaagtaactgtctggctttcccagctgctccagttctctctgcaaccc
ctgagccacagcctcctgggcggccgtagcacagtgatacaccgagttctggtggactgtccgtaaatgctttagaagatgatcgggccctagggcccag
ccaaccttccagccggtggcgctgaaggttttcccggcgctgccgatagtgatggtgcgctcccacatgcccggaaggctggcaatgcgtacatgttggt
tcccatcatacaccagccactcgtacacctcgtcactaaagcataagatattgtgccttgtgcagatgtcggcaatctcctccagctcctctttcctaaa
gaccttccccagggggttattaggagtgtttataacaagggctttagtgtgacggctgattttcccagccagctcttccaggtccagtctcccactccca
ctagtcatgggcact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: