XB-CLONE-3543778
Clone Name: IMAGE:7718197
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Gene:
aqp8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979842 [+]
cacaagtggggtagactaattggtaacctaaccaagtaataaggagacccatgattcttgaaagtgcccctgtagaaaggtctaaataagtagatccaat
acattgggttgtacattaataaggctgaaaagagagaggaaagatagaggaaaggaaagtgtgtaaggaaaagggagaagtaaaatgaagtgtagaagtg
ttctacagaaactgggtaggtaataaagtatgttgacttattgtggggtgaggcagagctgtcctaaggggcggccaataggggcagctggctgtagttc
agcacctccctgcaccaatagcaactcagagtgaagggagtggttttcttcaatatggaagcctcaaaatgggtaacttgcccctggcctctcaaccacc
aagaatcactcttagatgtgcagagaggaggtcgggaataagagaatgtggagtgagaaggaagttggggagagaaaggtggtgagaggaaatttgagag
tcagggaatgggagaggaaagggtggaaagcaatagattaaagagactgggatcttttaacataagggccaagatggagtaggtggaaggggtgaataag
atacaaaaggatattcataaggataataaagaaaagataggttaaagtgagtctatgagggaattacaaggttctgttgattaaaggactaaggaatctc
ccagtgggccccactgtgcagtttgtaccatagaaagtggtgtattacagataccagctagtgcatttgtactaactgtgcatagagagggcccctgata
gtcaggttctctggtgggcccagtccaacactgcctattattgtaaagtacagatagagaccgtatgagccagcatact
3' EST: CX979841 [+]ttagccatatgctgcaaaacatgcacaaagacccccagaacattccatcctttccttatttctaatacaaattaaaatcagctctacaaaataattacca
aataaagaaataccagcagaagtcaggaacaattaggccactgcctggtcctccctggcaccccccagttgcacccaccccgatggtgtatccagaggcc
cattagcaaaaacattgtccccaggcctcattctcataatgggatcactgccagcttcttctcttgcctatatccctccatgtaggatggtgtggactgc
actatttcttgtttggaaagagttaattctttgtacctatgggacctctcaaatattaggggttgtattgacttctttcagatttgtcagattactatca
ggatcattcagccaatctaacatctgttggaagcagaaatgtacaatgtgcagaattccatgccagcctattataatggctgaggatgctgggaatttgt
ggcatggcaggagttacctgccttttgctatacctgttgttgttgtatgagtgaatccggcatccccactgctattaagggcagtttttcaggggctttt
tccctgctataattattatgtatcttaattatagtacctttcaattatatagggaataatgtacccccttatgtataatataaggctattataagtccca
gatgacgtttatgaccatattaaagcacaaggacaaaggctttgacatcattaagcacttaatataacttataacatcaataagcaagttataaggatat
catttataggatattaaaggcttttctttattctcctttgcagggactggagggaggggga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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