XB-CLONE-3543767
Clone Name: IMAGE:7718007
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Gene:
gnl2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979849 [+]
taaagaggacactggtgtcagagatgaagcccgtgaggagatctataagaaaggccagtctaagcgaatctggggagaactctataaggtgattgattca
tctgatgtgattgtgcaagtcttggattccagagatcccatgggtactcgttcgccccatatcgaatcttatctcaagaaagaaaagccttggaagcatc
ttatttttgtcctaaacaagtgtgacttggtacctgcatgggctacgaaacgttgggttgccacattatctcaggaatatccaacattagcttttcactc
cagccttaccaactcttttgggaaaggagccttcatccaacttcttagacagtttggaaagcttcacactgacaaaaagcaggtcagcgtgggatttatt
ggatatcctaatgtgggcaaaagttctgtaattaatacactaaggtccaagaaagtttgcaacgtggctccattagctggtgaaacaaaggtttggcagt
acattaccctcatgcggcgtatatttctcatagactgccctggagttgtatatccatctggcgactcagaaacagacattgttttgaagggagtggtaca
agttgaaaagattaaaagcccagaggaccacattgctgctgtgcttgagagagccaagcctgaatacattaggaagacctataagatagagtcctgggaa
aatgcagaggatttcctggaaaagcttgcatttagaaca
3' EST: CX979848 [+]tagagtgctgctggcgctcttgccttctcctctcttttgatgaaagtttcttttgtggctgttcactatcttcttcatcggcatcacattgttttctttt
ctttccttttcctttatcttgttggtcctcacttggttttgtaaacaggttttctgtcagatgcttaggaattcttaaagcagaaaatcttttggctttg
gctttgtctaggaattttttgtactttgcaatccgatcatctaactcttttaccagtgctttagaacttctgtctttggtgcttgaatcctttgtttcac
ccattagcatatcactgctttcctcttctgtttcttcttctgcagagtcctcttcttcagactcaacatcagagatatctgggattccttcaataggtac
taaatcttcctctgagaattctggtgccacgctgatcttgccaaagttttgtcgtacatgggacatgagatctttgatctgatttctgtctggattttct
tgagaagcttctgttgtatcattttcctctgactgctccttagtaacactactggtatctgcaggagtagccaatgcctgtgctgtctcggaattatgtt
cagcattaggaggtttaacatagaacggaatcctccccctctgccagtcattaagaaccatttttgagacagtttgacggtctggttcaccgccctttag
aagttttcctgttctaaatgcaagcttttccaggaaatcctctgcattttcccaggactctatnctataggtcttcctaatgtattcaggcttggctctc
tcaagcacagcagcaatgtggtcctctgggcttttatcttttcaactgtaccactcccttcaaaacatgtctgtttctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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