XB-CLONE-3543764
Clone Name: IMAGE:7718055
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Gene:
dnajb6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979851 [+]
aaacagcaaacatcagtacaatgatccacttttagcaagcctctgacacagaagtgataagctgtacaaacatggttcatttaaaaataataattaaaaa
aaagaacataattggtatacacacagccggctacacctctagctgagaaataatagcagttgcctctagtgcaggtaacgaaatcagaggagcaaagtag
gcccattcagtcacagccgtcaattttatacattagaaaacattggtttgaaacaattgcttaggtgcaaaattgaagaaaaatatatagcattaaacat
tttacatcaatacatgtgagttataaaggggtttgtcatccctgtaagttataggaacagggaggaggaacagattagttaactgtaatcagggcctata
aagattaagaaagctagccactatggcacagcaatgggcacccactggtgtaaggaatacagcattccatgagaatccctgtaagttatagcaacaggga
tgagtaacaactgagtagactttaatcagggcctataaagattaagaaagctagccactatggcacagcaatgggcacccactggtgtaaggaatacagc
attccatgagaatccctgtaagttataggaacagggatgagtaacaactgagtagactttaatcagggcctataaagattaagaaagctagccactatgg
cacagcaatgggcacccactggtgcaaggaatacaacgttccacaaaaagccctataagtttaataaccacaggggtgagtaacagatgagttaatttaa
accagcaccccctacaagat
3' EST: CX979850 [+]ttgactcgggtttcagttcttcatttggttcttttggtggacatggtggtttctcttcattttcctcttcatcgtttggcggttcaggaatgggtaactt
cagatctgtatcaacctcaactaaagtagttaacggaagaagagttacaacaaagagaattgttgaaaatggacaagagcgcattgaagtagaagaagac
gggcagttaaagtccttaacaataaatggtgttgcagacgaagcagcattggtggaagagtgtgggcgccggggacagaatgcgctaccgtttcagccct
ccagcagcagcagtgccagaacctccaaaccccaccggcccgcaggccctcccagacacggccacagttacaaaagcgacgacgatgaggaggaaccaga
gaggatgaggggtacaagcaactgggagccccccttttactctgcaggacacaaagaagggagcaagagaaagaagcagaagcaaagagaagaatacaag
aaaaagaagtcttccaaaccaatctactaacgtggacatgggaggccttagatccttacaccagagagcaccgtattctatgggcgtaactttgacacca
tttttgttttgcttagatacattttttggaacgtagtcaacaactagggacttaaacttgttctatgtaaattctcaccagtgaccttccttctgtagac
tgaaggcaaatcctgtagataaaggaaagagctgctcttctctccttttaggtgatggcttcatgggatatagggtagattcctgagttgatttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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