XB-CLONE-3543717
Clone Name: IMAGE:7718251
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979882 [+]
cctgcttcgtttcgctgcgactccgagtccggcgcatgtgcagtagagtgaaaagccgactttggcacccccaggtgacttagcttttccttctccttta
aggccttttagcacaaataactctaatataaaaacactctgatcctcactgattttcatgcttacttaacccttgccctgttgccctttcatttaataac
cccatgctttggtggtccaaagtagatcagcttaccttataggcaatttatttagatataattgctcctgtggaaagacagtaaaatgatatctctctct
ctttgcatttgcagtgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactacaaagccaagtaattccctttgcaaagcaaatcc
atgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcagggtgaagatttctccttacgcagatgaagtaca
taaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaaagccaactagagaagaacactaacacaatcagt
cagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaagaatctttggcaccatattcagaaaaactccagg
cccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaagtgagagataagattggccatncagtacaaaagttacgtga
gagccctg
3' EST: CX979881 [+]ataaagtgcatgcatatcaaccacaacgtaaaacattacagggccttatgttgttactggggaggttcatcccagacataatgaccaagaaacaatggca
gcagaattataaataacatggaacattttagggctgtaagagaaacataaagctattcaggcacaacaaatgacagacataacacaaagacaaatctgtg
ttttacaaaacttgctacagtgtgcatttacttcacttttacattttcttcttacttgatttgcagtaggctgacagcccaatctctgggtatttttttt
ctttttatgattgatatggcatacaggtggtgcaagttgtaatggatactgtggtctgatgacaatttgggtgtatacgttgtttagttttcagtttcag
ttatacctttatttatgaagtcgctaaacttatctcggatgtctttttctaagtattccatttgatcttgcacagtcactgtgtatttcccaaatttcag
tttcatgtcctctaccctctgcactagtgccttgttgagtgcctctccatagggagtaatgctaactttaaattctgcaatcttctgctccatctgctgg
tttagctcggtgagatatggctctagtgtctgcttagcatccctagcatttacctggagtttctctttcagatcatctgcataggggctcagttgagctt
ttaactgctcagtgatctgttttaattgggtgtctgcttgctcaacactctttctcatttggaattccatgctctctatctgcttggctaaattctcctg
catctcctctgcataagggctcangctctcacgtaactnttgtacctgatggcccatcttatctctcacttcatcagtggtagggttta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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