XB-CLONE-3543700
Clone Name: IMAGE:7718205
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Gene:
fbxo32
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX979893 [+]
ataaattgcaaaatgcgcatctcacaaagtctcctgcaattattgcaccagatatacagattaatgacacgaggcagcgaggaggaggaggaggaggaat
tcaaacactcaataaacactaggaaattcgtctgcaccaaatctgtgctctttacagggaaacagagtaaacaccagcaacttcagataaatatatatat
tttttgcctaacacaggcctaatcagttctatgtagttctcttcatcttcatataatgatcccatttaaaaagtaccgttgttttgtttgctttatattc
agcccctactgctctaggtgtttatatgtctggggtgtcccatcccaagctaacaatgttttcatccttttcatttcattcccagttgtctccccgttcc
ataagggacttaaatattcacttcacttctaatgggcatatttattatagtgtgtaagtcaacaacatcgctgatgctgcccatagtaaccaatcagcaa
ttagatttaaacagtcagctacaaataacgtttatacaatattgaattgtttaactcatccaccacccctttagtttgcaggctggtgttgccttatggg
gaagccatagctgacacaaaactacacatttggatcccgtacttctgtgtatagtgattataggtgcagagctggctccacttacagtaaccctgagctg
tccaactggaggcctgtgggccagatgtcgccctcc
3' EST: CX979892 [+]caaaatggctgtaaatagagactatgttggaaaggatttgagctgctccggatggagcaatatgtgctataaatattggacttctttgtgcagaattact
tcaggcaataccactaatagagccaaggttccgtttttggtgatagtaccttcatacgtgtaagtatattattggtcactgtgatcaagggctttacact
gtatcccaaatgcaccaggtttgctcgaccccgctaatttcttcataatctgcaggtgaatattagtggggtgcggcttccctcgcccctctatcatgtt
agcgtgggttccaaagctgcctcaggccttgagaagggtgtcggccctgaccgtcacattactaaccagtatgaccactgtgtaaatgacttcatagcag
tcctgcgccaagatgtaccggcgccaaaggtgcaataatgatgtcatacagtaaagtctatgaagcctttaagcaaactgggggccatacaattttggag
ggcgacatctggcccacaggcctccagttggacagctcagggttactgtaagtggagccagctctgcacctataatcactatacacagaagtacgggatc
caaatgtgtagttttgtgtcagctatggcttccccataaggcaacaccagcctgcaaactaaaggggtggtggatgagttaaacaattcaatattgtata
aacgttatttgtagctgactgtttaaatctaattgctgattggttactatgggcagcatcagcgatgttgttgacttacacactataataaatatgccca
ttagaagtgaagtgaatatttaagtcccttat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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