XB-CLONE-3543432
Clone Name: IMAGE:7718087
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Gene:
cela3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980076 [+]
aatggtgaaagtgccaaaccctacagttggccttggcaggtctctctgcaggtcctgataaatggggtatttatacacaattgtgggggaaccctcatcg
ccgacagatggattctgactgccgcccactgtatcaacttctccaggaccaatcgggccgtggttggagattatgacttagccaatgaggaaggagcaga
ggagatcttccttattccatctgaggacatgtttgtgcacgaatcatggaacaataattgtatcccttgtgggaatgacattgcccttatccgattgtcc
cgccctgtgcagataagtgataaggttcaactgagctgcctcccccccgccggggaactgctccccaacaatttctcctgctatgccagtggctgggggc
gactttacactggggggccctatcccgatatcctgcagcaggcgctgctcccggtggtggattatgatcactgcagccagagagactggtggggcgccac
agtgaaacgcagcatggtgtgcgccggaggggacattcgctctgtctgtaatggtgactctgggggacccctcaactgccagggggccgatggccggtgg
tacgtacatggggtgaccagctttgggtctggctacgggtgcaacaccctcaagaagcccagtgtgttcactcgcgtgtcggcctttaactcttggatcc
agcaaaccatcagtgagaattccgtaagtggattccagtagatcctttccaggaatctgggcagagtcatctcttctnat
3' EST: CX980075 [+]gaattgctgaggatttattggatctgtgttctggagaatcagtacaaggaccaatagaagagatgactctgcccagattcctggaaaggatctactggaa
tccacttacggaattctcactgatggtttgctggatccaagagttaaaggccgacacgcgagtgaacacactgggcttcttgagggtgttgcacccgtag
ccagacccaaagctggtcaccccatgtacgtaccaccggccatcggccccctggcagttgaggggtcccccagagtcaccattacagacagagcgaatgt
cccctccggcgcacaccatgctgcgtttcactgtggcgccccaccagtctctctggctgcagtgatcataatccaccaccgggagcagcgcctgctgcag
gatatcgggatagggccccccagtgtaaagtcgcccccagccactggcatagcaggagaaattgttggggagcagttccccggcgggggggaggcagctc
agttgaaccttatcacttatctgcacagggcgggacaatcggataagggcaatgtcattcccacaagggatacaattattgttccatgattcgtgcacaa
acatgtcctcagatggaataaggaagatctcctctgctccttcctcattggctaagtcataatctccaaccacggcccgattggtcctggagaagttgat
acagtgggcggcagtcagaatccatctgtcggcgatgaggggtcccccacaattgtgtataaataccc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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