XB-CLONE-3543323
Clone Name: IMAGE:7718146
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Gene:
ctrc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980149 [+]
cagggaaccagtggtgcttggggtcacacctgcggcggaaccctcatctctgaacagtgggtcctgactgctgcacattgtatcagctctggccgtgtct
acagagtgttcctgggcaagcacagcctgcagcaggatgaggctgaagccgtcgctatctcccctgagaaaatcattgtccatgagaagtggaattccct
tttcattatcaacgacattgctctgatcaagctgtcccagcctgctcctctgagcgaagcgatccaaccagcctgcattccttccagcggtgccatcctc
gccaacgacttcccctgctttgtgactggatgggggcgcctctacaccaacggaccaatcgctgacaacttgcaacaagcccttctgcccgttgtggacc
atgccacttgcaccctgcgtgactggtggggaagccaggttcagaccaccatggtctgtgctggtggtgatggaattgtgtctggctgcaatggtgattc
tggtggccctctgaactgccaggcagcaggtggagcttgggaagtccacggcattgtcagcttcggctctggcattagctgcaactatgccaagaaacca
accgtcttcacccgtgtgtccgcctataacgactggatcagcgagaaaattgccagcaattaagaacaagcgcaatttccatcgtatccgcaacaacagg
gcatctgattcgtgcattcctcgtatttcctttaacacctcgtgtcatacacattttggatttc
3' EST: CX980148 [+]gaaatccaaaatgtgtatgacacgaggtgttaaaggaaatacgaggaatgcacgaatcagatgccctgttgttgcggatacgatggaaattgcgcttgtt
cttaattgctggcaattttctcgctgatccagtcgttataggcggacacacgggtgaagacggttggtttcttggcatagttgcagctaatgccagagcc
gaagctgacaatgccgtggacttcccaagctccacctgctgcctggcagttcagagggccaccagaatcaccattgcagccagacacaattccatcacca
ccagcacagaccatggtggtctgaacctggcttccccaccagtcacgcagggtgcaagtggcatggtccacaacgggcagaagggcttgttgcaagttgt
cagcgattggtccgttggtgtagaggcgcccccatccagtcacaaagcaggggaagtcgttggcgaggatggcaccgctggaaggaatgcaggctggttg
gatcgcttcgctcagaggagcaggctgggacagcttgatcagagcaatgtcgttgataatgaaaagggaattccacttctcatggacaatgattttctca
ggggagatagcgacggcttcagcctcatcctgctgcaggctgtgcttgcccaggaacactctgtagacacggccagagctgatacaatgtgcagcagtca
ggacccactgttcagagatgagggttccgccgcaggtgtgaccccaagcaccactggttccctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: