XB-CLONE-3543277
Clone Name: IMAGE:7718198
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Gene:
rsu1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980181 [+]
ctgaagaagatagtggaggagagcagggagaagaatgtgcctgacattgatatgtgcgacagggggatcgctaacatgctggatgtgccgggcctcttca
ccttatctcacatcacgcagttaattctaagccataacaagctcacaactgttccaccaaacattgccgatttaaaaaatttggaagtgctcaacttttt
caataatcagattgaagaattgccaacccagatcagcagccttcagaagctcaaacacctcaatcttggtatgaacaggttaaacagtttaccacgtggg
tttggctccttgcctgctttggaagtccttgacctgacctacaacaacatgaatgagaactccctgcccggaaatttcttttatctaaccaccctgcgcg
cactctatctaagtgacaacgattttgaaatcctgccgccagatatcgggaagctcacgaagttgcagataatcagtcttcgggataatgacctgatatc
attgcctaaggaaataggggatctcacccagcttaaagagctacatatacaaggaaataggctaacagtcctgccccctgaacttggcaatttggatctc
acagggcagaagcaggtgtttaaagctgagaacaatccttgggtcacgccaattgctgatcagttccaagtgggagtgtctcatgtctttgaatatatcc
gctcagaaacctacaaatacctgtatgggaggcacatgcaagccaaccccgag
3' EST: CX980180 [+]attgattcagtcaatactcaacaggttcttaactgggaattatccgaacattcccattcaccacataggcatgaatcaaggctgatctttacaatctgca
caaagccactgtatgagtgatcctttctttaaagggatactgtcaagatttttatggggtacttttatttctaaattacactgtttacatagcaaataat
tcactctaccatttaacatgtttagctgtaatattggtgtgtaggcgccatctcagtgcattgtgcctgagtctgagctttcagaaggagccagtgctac
acattagaactgctttcagtaacctattgtatctcctactcccatgtaactggaggagtcccaagccagacttgaatttcttactattgagtgctattct
gatacctactgggagctgctatatttataaagtgggttgcacctgtgaaacaaaaagaatatacaggtgtatactccttctataggagaatgcagaacat
taagattaaagggatagaaaactacacggctattttggtattaaaatgaagtcataaagtaaaatcagaggggtatattagtttcttcgaggccttgaac
acgttggcgttggcacaacattgctgcctggtgcaacagtctgactgtatgatagaaatgcaactatgcaactgccctaggaatgcacgcaatacaatgg
ctgatgaataaaactggacccatgtttaagtcctttcatgttagagttaaagacattacagaaattcaatacaaaaaaaacataaatgtaattaaaagtg
acaagcggaacatggtttatgccagtgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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