XB-CLONE-3543254
Clone Name: IMAGE:7718248
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Gene:
adgra3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980196 [+]
gatggtggacatagcaagtaatataatgctggccgacgagcgtgtattgtggatggctcagaaagatgcaaaagcttgcagtcgaatagttcagtgcctg
cagcgtatcgctacctatcgattggccaatgcggttcaagtttactccacatactctccaaacattgctttggaagcatatgtgatcaaatctgctctct
tcactggaatgacctgcacagtttttcaaaaagttgctgccgcagaccgtacagtgcatgctgactttggccgacgagatctagatggcagcctggataa
acaactaagcttcaagtgcaatgtgtccaacacctttagcagcttagcattaaagaacactgttgtggaggcatctattcaacttccatctgcacttgtg
ccaaaagagaaacgagaacgcagagcaatggacgagtctttctacaagcttcaactaattgcatttcggaatggaaagctcttcccaacagcaggaaact
cctcacaccttggtgatgatggaaagaggcgaacagtggtcacacctgtcattcttgccaaactagatgggccgaaccgaaatattcccagtgtccctat
taatataactctacggcgctttgctaacgggaaggatgcagttgcagctcagtgggattttgatctgcttaacgggcaaggtgcatggaaatcagaaggg
tgccatataattgcatcagatganaacataaccaccatccagtgctcctctctcagcaactatgcggtactaatggaccttacaggaacagaagtataca
gtcacccagtcacccctctgcaccc
3' EST: CX980195 [+]gcacttttatttgtgcaagaagactctgcgctgctgtttgtacattttggcgcatcaccattagtcccgttagaattagatggcggaacattaacttgca
cagaatatgtactcctcccggggcagaaagtgttcattaaagcacgtctcaagtcttctctgttcatgcaatggtgtacaactaaaaaggcaccaaggct
taaacaacttacaccaaagaagcagctgaataccaagtccatggggtaatacaaagagacggccaaagccccaaatatccagagagacacaaagagaaat
aaagtaaggctggcaccaagaagctgcgcctgaaaggtctgctcattctctaaagctgaagtagacaccaaggacaccgagagggagtcttgctgattcc
cttcaccactttcacttgcggccaatcgctgctgctcctctgtctgttcttttagctcaaacactctgtcaggatggcgcttcagctgaatgaatatact
caggaagtacatacaattgataaacacgataaaacttgcaggaccataaaacgcacccagacttggttcccatgccatccagcagtagggggcgttgggc
tggcttccatagttttttatattcgcagctgctgttatgccgcacacaattatagggattcctccaccaatgagataaaacctgagcattggccttgggg
gagggggcaagtcatctgggtcctggcatcgcttggcctttttggtgacttgcttatatatgttgcgagcagttactcccacccaaagtacagtagcaag
agtgggatagtgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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