XB-CLONE-3543197
Clone Name: IMAGE:7718062
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Gene:
creg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980234 [+]
aagtgctgaactgggtcgcaatggctggaaaatgacgcaaagctacggagtcgtatgcagaaagccaatagtgcgtagggggtgttcccgaaactgattc
tgttgtaccatgtgactaattattcaaactactccctgattgctaaggtaatgtggaccctagcaaccagatagcggctgaaactcaaaactggctgcag
aacgtccaacgaaataactaaaaaagtccaaataaaaaatgaagaccaattgcaaatagtcagagaatattactctctacatcatactaaaagttaactc
caaggtgaacatccctggacctgttatccagaatgctcgggaccaagggtattccggataaggggtctttccgtaaatcggatctccataccttaagtct
actaaaaaatcaataaaacattaattaaacccaataggatggttttacattcaataaggatcaattacaaggtactgttttattattacagagaaaaaag
gaatcagtttgaattatttgattaaaacggagtctatgggagacaggctttctgtaattcggagctttctggataacgggtttccggattagggatccaa
ggtcggactgggtgggcccccaaccctagtgggccccgacggcccagtccgacccttgccggagctcctactactgactgttcctcccctgacgcgcacg
gggagaaagtcaggtgggagacgggtgggcgggagccccggtgggtgtgagaaaggactaatacacaaagtaaatatggtgtatctttaatcttttacta
actgcattttagaatgcttttctt
3' EST: CX980233 [+]gcgagaggcaagtttttctgacctttgaagtccagtttgaagattgtatatcttgatactgggattataggtcttgctattgtttttgtatccaatatct
atagcagaaaaggtagggcatataatgcattacactaatgtactaggggacttttgaagcctatgagcttaaaatcactttcctcttaaaactattgaaa
gcccatctgtcttgctatgggatattttagaatgtagttcattattgctaagaaaagcatttctaaatgcagttagtaaaagattaaagatacaccatat
ttactttgtgtattagtcctttctcacacccaccggggctcccgcccacccgtctcccacctgactttctccccgtgcgcgtcaggggaggaacagtcag
tagtaggagctccggcaagggtcggactgggccgtcggggcccactagggttgggggcccacccagtccgaccttggatccctaatccggaaacccgtta
tccagaaagctccgaattacagaaagcctgtctcccatagactccgttttaatcaaataattcaaactgattccttttttctctgtaataataaaacagt
accttgtaattgatccttattgaatgtaaaaccatcctattgggtttaattaatgttttattgattttttagtagacttaaggtatggagatccgattta
cggaaagaccccttatccggaatacccttggtcccgagcattctggataacaggtccagggatgttcaccttggagttaacttttagtatgatgtagaga
gtaatattctctgactatttgcaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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