XB-CLONE-3543075
Clone Name: IMAGE:7718216
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Gene:
eif3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980315 [+]
aagaaactggaagagcaagaacgcaagaagcgccagcgagagctcgaaattgaagagcgggaaaggaagcgcgaggaggaaaggcggggcggggatgata
ctttcaggaaggattcttctcgctggggagaacgagaggaaagtggatggagaagaggtgcagacccagatgaacgcaagcaggttcctccagaaaggga
ctggcgtaggggtgggcctgattcgaagccggtaataaatgaagatgcctcaaacagagaggaagatgagaatgccgctttgaggaaggatgaagaacag
gtgtctagtcgtgcctttgaagaaaaggtttcactgcccgatgctgatgaagaaaaaggaggttcttggcgagatgaagatcgtggtccaaaacggggtc
ttgaagaagatagaggacccagacgtggtattgatgatgcaggccctaggagaggatttgaggaggacagagggccacgccgtggaatagaagatgacag
agctccaaggagaggctttgatgatgacagaggccctaggagaggctttgatgatgatagaggtcctagacgcggctttgatgaagaccgaggcccaagg
cgtggcatcgatgacgatcgtggtcctagacgaggctttgatgaagatagaacaccacgccgtggctttgatgatgaccgtgggccaaggagaggatttg
atgatgacagaggccctaggcgtggctttgatgaagatcgtggtccanggagggggcttgaggatgatcgtggtccaaggagaggctttgaggatgaccg
cg
3' EST: CX980314 [+]agcagcaacaagtcctgtgttggccgaaatgcgttgccgtacagcacaaccggatgttccttaaccctgacagagtaatgcagctttttatgagaaaaag
atatagaaagggtaaaaattcctcctactacactcgctttatttaaataggacatttccatagagactaaaaaagaaagaaaaaaaaaaagtgatagttg
ggcgacagtcgtagcaaaatgtcagccccgacactgcactgtagcacgcgactcatacaggtgtaatatggagccaacatgcagcccctgcagcttcaac
atcccagcacccagcctttggatgatgggagttgtagttttccatctggggtaagtacaagggtcacttagctttattacaaaccaatgaagtagagcag
gctttgcgttgtgggcagcgatacagagagagaagggggggggggtcgccttcaggatcatgtgacctcacctttagcctttaacctggttatattttac
aactgaggtatgagtaagtacattggcctgaaaaataagaagctttcgtttcattggatgaaacagcgtgaccctaacaatccactgtttctctgtacaa
gtggcagaggctgcgccgccctgctggccataaggttaggctactgggacagaatgggacccagcctgggaggttggtacagcgcagggctgtccaagtg
gaagcctgtggggccctcaaagagatccatatggacccccccaaatattctattgtgtagcagcgcccaaaaacactgatttggatttcagtgattgggg
atgggagggctgcaggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: