XB-CLONE-3543054
Clone Name: IMAGE:7718170
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Gene:
dedd2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980329 [+]
tttctttgaaagcctaatttttaacttttttttttttttaaataaatccttgcttgaatttatgctttataataaatcctaatttaagtttccacttaaa
tacagggatgactggcaaaaataacacaactgtatgtatgaacattcaggaaggaacttttttgaatgaaaatctgtaatttaagaccaaaaacatatcc
gcagtccacacgtttagcgctagtgacattaaggttatttgttcctttttcgtggcctcgcaatttcttaataagaaaaggacatggaatgctaggctta
actttaacacacttctaaccaactttttaggttttctagacctaagccgaatacttttgggtacacattgtattttcaatggttaaatgaagtcatttta
gttctattatatagtatatggatatattgctatcacttctcttgctaatcagggatgcaacaaatccaggattcagtttgggatccagctgaattatagc
attcagctgatcagttgaggacaactgtttttgtcccagttaagccaattttgaagcattacgctcaacctggaatatgcatatcagctatcttatttgt
atggtctgaatctttcaatttggcttttggcaaaactgaaaatgatggatttggtgcacccctattgctacataactacagaaagaatgtaatatgattg
atcataactatatttctaaaatatgattcttttgtgggcttttcaagtgaatgcaaaattactatttagctactacataaaataacacaatgcaattttc
tttttaaatttagtcatttcaaaaatagc
3' EST: CX980328 [+]ttgtatgctatagtttgaatttttgggggattgttagctttttctaacctaaatatatatttagtaaattttgtaaattcttgtgtaattaatatcggga
tttacaaatatttctgttctgatgcttagcgcagcagataccagaaatatagggttatgtttaagcatttgtcctaaaattactgtaatgatattggtat
caatgtttctcttatggatccattaataactctcattattatcagacatttaccacatagggcttgcagtattctttgtgaattctgtaagcagcttcaa
tagaattacaaatccagagctggacccattaagcatttctgattgatgactcctactttaaaatgtttgggatccttagtggtgactgttcataacagac
ctcatacttcaagttatgtgacagatgagcaggctgtcactggcagaccttattttctgatatctgctgatcctcaacatatttagttcatttagcggga
aatttcatattcaaataaatatgttcttacattgttaaaaacattacttgctacctattagaaaagtgctgtgctgtaaatgtcaaaagctcaatctgtc
tctctcggttcacagttaagatcgccaatggaaaaaagccttaactagctgctctgacagtctctggcactgcaagtcaatggtaaacttgggagcaaaa
cacaggcttgtttgtttctgcttccgtctgtgctctatatacgacctgctcaacattttacaataagcatctcaacatggacaattatgttacgaatatt
gca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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