XB-CLONE-3543010
Clone Name: IMAGE:7718126
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980358 [+]
gaacttcacggtagaccagattcgttccatcatggacaagaaggcgaacattcgtaatatgtcggtcattgcccatgtggaccacggtaaatctactctg
actgactcactggtatgcaaggctggtatcattgcttctgctagggctggtgagactcgttttacagataccagaaaagatgaacaagagagatgtatca
ccatcaaatcaacagcaatctctctcttctatgagctctctgagaacgacttggcttttatcaagcaaagcaaagatgggtgtggcttccttataaactt
gattgactcccctggccatgtagacttttcctctgaagtaactgctgctcttcgtgtcacagatggtgccttagttgtagtggactgtgtttcaggcgtc
tgtgtgcagactgaaaccgtgcttcgtcaagccattgcagagcgcatccgccctgtgttgatgatgaacaaaatggatcgtgctcttttggagctgcagt
tggaaccagaggagctctaccaaacttttcagcgcattgtggagaatgttaatgtgattatttccacctacggtgaaggagagactggacccatgggaaa
tatcatgattgaccctgttcttggcacagttggttttggctctggtctgcatggctgggctttcacactaaaacagtttgcagagatgtatgtcgcanaa
tttgcagcanagggggaaggtcagttggctcctgcagaacgggccaaaanagtggaggacatgatgaagaagctgtggggtgataggtattttgaccctg
caaatgggaagttcagcaagtctgcaacaaacgctga
3' EST: CX980357 [+]tgcagtacatctttgctctacattctgccctcgttcctgtcaaccctcaaattcacatttacttccccagccatccaagatgttttcagagagcagaagc
tacccatcaagaataaatagaaagttatgatcatgccaatgaaagattatattcagggttccaacagaatgttgcagggaatgctgacatcttttgggta
tagttcatttccacttaatcaatgctgacaggagtaaagcaaaggtttacagcttgtcaaggaagttatccaaggcttgaacaccctctttcaagccctt
cctcttccgggtctctgccaccacttggaaaggcctggtggagttatcgaaaggatctccaggtagaatttgccagtggtcaaacacacactgagggaag
gcctggccaccagtgtttgatcttaagtcagcagtgaaaccaaatgactcatttacaggcaggtatgccttaaccacaaacattggtgttccagccacct
gggattcttcaaagacatgcccacgctttctgttcaagacaccatagatacctcctaccacctgctcagggcactgaatttcaacaaggtagattggttc
catcagacgtggctgagcagtcaacacacaagcatacaagacgcggcgtgcagttggaatgatctgtcctcctcctctatggatggcatcagcatgaagt
gttacatcatggacatcaaaacgaacacctctcanattttcttcacacaggacaccctcctttgtagcccactggaatcctgncacaacgctgtctttga
tttcattcagatactgaactcccttaggtacatcagtaagtatgttgggcccagagccatccaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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