XB-CLONE-3542937
Clone Name: IMAGE:7718276
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Gene:
pno1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980407 [+]
gaagagccagacaagtctgagccaaccagctggttctttgatgtaaatgttgccagagattctgccaatgagatcttctcttctgcacgccgtttcattg
cacataggtagatgtaagtggggccacattgataggatgttcagtcgttccacttcacgcaatgttagaacctatcggcagcgcgacttgccactgcccg
tatgttgccgtacactttggatggaggacttcctaagataaggttacagattgcagtcctggccatcttgatgttctgaaaggatcccagtatgtgtatt
tttgaatcagcaagaactattcttgtccttgtgacattttctatggtgaactttgtcttgcctccttttccagctatccgcccaatggcccgagacaagt
ggtctcccttcagcggtttaacatctgtgacttcaaatgattccaagaagagatcgtcgagccggaccagggctaacgcatcctcaacctgaaaccccag
aataaaggctctgacaaaatcagttgcttttgttaatgcgccaacatctgtggtttccttgcaagtctttatttctacatttcttgtctttaggttgaac
cgcacttgaagctgtaagtgttccacaatcggagtgaagattttcatccagttttctttaagaggagtgtacctgtgtgaggngacggggactttcctca
tttcatctttgtctcccatcagcttatccccagagatgggaggaaagtccggcctcttactcggctgctccccggtgtccatgggcctctcctcgggcgc
tcttctcttcctggtttttttagaggtgacacttgtgaaactttccccggaggctgcggcg
3' EST: CX980406 [+]gtttttttctatcgcttctgtttggaataaatcacatcgcaagaatggagaccgaaagcgccgcagcctccggggaaagtttcacaagtgtcacctctaa
aaaaaccaggaagagaagagcggccgaggagaggcccatggacaccggggagcagccgagtaagaggccggactttcctcccatctctggggataagctg
atgggagacaaagatgaaatgaggaaagtccccgtcccctcacacaggtacactcctcttaaagaaaactggatgaaaatcttcactccgattgtggaac
acttacagcttcaagtgcggttcaacctaaagacaagaaatgtagaaataaagacttgcaaggaaaccacagatgttggcgcattaacaaaagcaactga
ttttgtcagagcctttattctggggtttcaggttgaggatgcgttagccctggtccggctcgacgatctcttcttggaatcatttgaagtcacagatgtt
aaaccgctgaagggagaccacttgtctcgggccattgggcggatagctggaaaaggaggcaagacaaagttcaccatagaaaatgtcacaaggacaagaa
tagttcttgctgattcaaaaatacacatactgggatcctttcagaacatcaagatggccaggactgcaatctgtaaccttatcttaggaagtcctccatc
caaagtgtacggcaacatacgggcagtggcaagtcgcgctgccgataggttctaacattgcgtgaagtggaacgactgaacatcctatcaatgtggcccc
acttacatctacctatgtgcaatgaaacggcgtgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: