XB-CLONE-3542931
Clone Name: IMAGE:7717990
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980411 [+]
aggctcccaagccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgcaaagcagcaccaaggatacggtggaccagatc
cagcagtcagatatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactacaaagccaagtaattccct
ttgcaaagcaaatccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcagggtgaagatttctcctta
cgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaaagccaactagagaagaac
actaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaagaatctttggcaccatatt
cagaaaaactccaggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaagtgagagataagattggccatcaagt
acaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagcatggaattccaaatgagaaagagtgtt
gagcaagcagacacccaattaaaacagatcactgagcagttaaaagctcaactgagcccctatgcagatgatctgaaagagaaactccaggtaaatgcta
gggatgctaagcagacactagagccatatctcaccgagctnaaccagcagatggagca
3' EST: CX980410 [+]gcattgctgggttgcattttattgagaagcattgataaagtgcatgcatatcaaccacaacgtaaaacattacagggccttatgttgttactggggaggt
tcatcccagacataatgaccaagaaacaatggcagcagaattataaataacatggaacattttagggctgtaagagaaacataaagctattcaggcacaa
caaatgacagacataacacaaagacaaatctgtgttttacaaaacttgctacagtgtgcatttacttcacttttacattttcttcttacttgatttgcag
taggctgacagcccaatctctgggtattttttttctttttatgattgatatggcatacaggtggtgcaagttgtaatggatactgtggtctgatgacaat
ttgggtgtatacgttgtttagttttcagtttcagttatacctttatttatgaagtcgctaaacttatctcggatgtctttttctaagtattccatttgat
cttgcacagtcactgtgtatttcccaaatttcagtttcatgtcctctaccctctgcactagtgccttgttgagtgcctctccatagggagtaatgctaac
tttaaattctgcaatcttctgctccatctgctggtttagctcggtgagatatggctctagtgtctgcttagcatccctagcatttacctggagtttctct
ttcagatcatctgcataggggctcagttgagcttttaactgctcagtgatctgttttaattgggtgtctgcttgctcaacactctttctcatttggaatc
catgctctctatctgcttggctaaattctcctgcatctcctctgcataagggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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