XB-CLONE-3542895
Clone Name: IMAGE:7718232
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Gene:
ada
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980435 [+]
ctataggccatccataacaaagtaggaatataatgcaatgtagatactgtatgactgtgattggattgactgtacctgcaatttaaaattcaaacccaag
gctccaagtgcggctataaactgcattagggatttgataggtttggttgctaaggctggggatcttggatggacttttgcatgtgtgtggaagcccgtgt
agatatctgtgagcattaaccaaacggcagcaatatgcgggggggttcttaaccaatatgaaaccaaagacaatttaatacttgcatcaaaattaagact
gcttatttattttcttcatatatttccatatatttcatgagaaacctgtggatgtttggtacatttgagaggtgtcgacctttatgtgcaaagaaaccga
aatatgtattttcatttttggtttttagattatgagcaggagaaaatgtgtgcatcttcttcccgttcattgactccagacttatctttccatgatacta
ataagcattaaggcggagctcaggctttattggattagagtgcagaagcccaacctcatgcatagaatgtgtacaattaataaagcaaatatatgagaca
gagccttcagtaggtcgctgtgaatgttgtgttgttggtagatacattggtcaagctgaacatggagaactgttcccataatgccttgctaggtttgctg
taatactgtatgtaacccagttgggggtgtatcctgtttagggtgtactcagaatac
3' EST: CX980434 [+]tacactgagcactatggagagcaagggcttttaacaaaccaaaggtagagctccacgttcacctcgatggaagcatcaaaccagaaactataatacattt
tgccaaaaaaagacaaatcaaactccccgcagacacggtggaaggcctcctagagcacgtttcctacaaagaacctctaagtctgacggaatttctcagc
aaattcaatcattacatgccggccattgcaggtgacagagaggctataaagagaatcgcctatgagtttgtggaaatgaaagccaaagaaggggtgatct
atgtagaagtccgatacagcccccatttcctagctaactccaaagtggaacccattccatggggacagaaagaaggtgacattacgccggatgaagtggt
tgatctagttaaccagggattacggaaaggagagaaagcgttcaacatcaaagccaggtctattctatgctgcatgcgacacatgccaagttggtccaca
gaggtggtcgagctttgcaaaaagtaccaaaatgacactgttgttgccatagacttggctggggatgaatctctcaactgtgaatcgtacccggggcaca
ggaaggcgtatgaggaagcagtcaaatgtggaatccatcgaactgttcatgctggagaagttggaccttccagtgtagtgaaggaggctgtggaagtgct
gaaggctgaaaggattgggcatggctaccacattactgaagacccaaatctatataaagagcttctgggaaagatatgcactttgaggtgtgcccgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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