XB-CLONE-3542808
Clone Name: IMAGE:7718479
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Gene:
yeats4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980492
[+]
cttcaatgtgggtttgtagctttctgcagtgagctgtgcgtgttcgactgtgaactgccccggcccctccccggccgcctcccgctgcaatatgttcaag
aggatggctgaattcggcccggactcaggagggagagtgaagggcgttactattgtgaagccaatagtgtatggaaatgtggcccgttactttggcaaga
aaagggaagaagatggccatacccaccagtggacagtttacgtgaaaccatatcgtaatgaggacatgtctgcatatgtgaagaaaatccaatttaagtt
gcatgaaagctatggaaatccattaagagttgttaccaagccaccttatgaaattaccgaaacaggatggggtgaatttgagatcatcataaaaatcttt
tttattgatccaaatgaaagaccagttacactctaccatttgttgaagttatttcagtctgacacaaatgcaatgttaggaaagaagacagtggtgtctg
agttttatgatgaaatgatattccaggatcctacagctatgatgcagcagcttctaacaacatcacggcagttaacacttggtgcatataaacatgaaac
agaattttcagagttggaagcaaagaccaaagagaagatggaaagtgcaaagaagaaaacaagctttgaaatatctgaactgaaggagaggttaaaagca
agccgagaaacaataaactgtttaaaaaatgaaattaagaaacttgaagaagatgatcagactaaagacatttaacttttttccctgttatgttttgtat
agcattctcctggtactgtttttggtccatttttttctgcag
3' EST: CX980491
[+]atagggcgagaggcctgcagaaaaaaatggaccaaaaacagtaccaggagaatgctatacaaaacataacagggaaaaaagttaaatgtctttagtctga
tcatcttcttcaagtttcttaatttcattttttaaacagtttattgtttctcggcttgcttttaacctctccttcagttcagatatttcaaagcttgttt
tcttctttgcactttccatcttctctttggtctttgcttccaactctgaaaattctgtttcatgtttatatgcaccaagtgttaactgccgtgatgttgt
tagaagctgctgcatcatagctgtaggatcctggaatatcatttcatcataaaactcagacaccactgtcttctttcctaacattgcatttgtgtcagac
tgaaataacttcaacaaatggtagagtgtaactggtctttcatttggatcaataaaaaagatttttatgatgatctcaaattcaccccatcctgtttcgg
taatttcataaggtggcttggtaacaactcttaatggatttccatagctttcatgcaacttaaattggattttcttcacatatgcagacatgtcctcatt
acgatatggtttcacgtaaactgtccactggtgggtatggccatcttcttcccttttcttgccaaagtaacgggccacatttccatacactattggcttc
acaatagtaacgcccttcactctccctcctgagtccgggccgaattcagccatcctcttgaacatattgcagcgggaggcggccggngaggggccggggc
agttcacagtcgaacacgcacagctcactgcagaaagctacaaacccacattga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: